Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Makioka, A.; Stavros, B.; Ellis, J.T.; Johnson, A.M.
    Detection and characterization of DNA polymerase activity in Toxoplasma gondii (1993), Parasitology, 107, 135-139.
No PubMed abstract available
Search for more literature for 2.7.7.7

Inhibitors

Inhibitors Comment Organism Structure
2',3'-dideoxythymidine 5'-triphosphate
-
 Toxoplasma gondii
K+ optimum concentration 50 mM, inhibition at higher concentration Toxoplasma gondii
additional information enzyme is not inhibited by cytosine-beta-D-arabinofuranoside 5'-triphosphate which is an inhibitor of alpha-polymerase, monoclonal antibodies against human DNA polymerase alpha do not bind; no inhibition by aphidicolin Toxoplasma gondii
N-ethylmaleimide
-
 Toxoplasma gondii
Salt sensitive to high ionic strength Toxoplasma gondii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulates at 50 mM, inhibition at higher concentrations Toxoplasma gondii
Mg2+ required Toxoplasma gondii
Mg2+ optimal concentration: 6 mM Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 sedimentation experiments Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Toxoplasma gondii
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
 Toxoplasma gondii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Toxoplasma gondii diphosphate + DNAn+1
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Toxoplasma gondii