General Stability | Organism |
---|---|
unstable in solutions with low ionic strength, glycerol and bovine serum albumin stabilize | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoic acid | - |
Bacillus subtilis | |
Ara-CTP | polymerase II and III | Bacillus subtilis | |
hydroxyphenylazouracil | inhibits only polymerase III | Bacillus subtilis | |
KCl | - |
Bacillus subtilis | |
additional information | no inhibition of polymerase I by ara-CTP | Bacillus subtilis | |
N-ethylmaleimide | polymerase I and II; polymerase III | Bacillus subtilis | |
single-stranded DNA | - |
Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Bacillus subtilis | polymerase I plays a role in repair of chromosomal damage | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Bacillus subtilis | polymerase III is necessary for DNA replication | diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
polymerase I, II and III | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.042 | - |
polymerase III | Bacillus subtilis |
0.4 | - |
polymerase II | Bacillus subtilis |
Storage Stability | Organism |
---|---|
-60°C, stable for 60 days | Bacillus subtilis |
0°C, 3 weeks stable in 0.5 M potassium phosphate, 2 M ammonium sulfate or 4 M NaCl | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | template specificity polymerase I, II and III | Bacillus subtilis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | polymerase I plays a role in repair of chromosomal damage | Bacillus subtilis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | polymerase III is necessary for DNA replication | Bacillus subtilis | diphosphate + DNAn+1 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
heat stability of polymerase I, II and III | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
hydroxyphenylazouracil | pH 7.5, 37°C | Bacillus subtilis |