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Literature summary for 2.7.7.7 extracted from

  • Lehman, R.
    T-phage polymerases (1981), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 14, 51-65.
No PubMed abstract available
Search for more literature for 2.7.7.7

Activating Compound

Activating Compound Comment Organism Structure
thiol stimulates Escherichia phage T7
thiol required Tequintavirus T5

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide great sensitivity of phage-induced enzyme, relative insensitivity of pol I Escherichia phage T7
N-ethylmaleimide great sensitivity of phage-induced enzyme, relative insensitivity of pol I Tequatrovirus T4
N-ethylmaleimide great sensitivity of phage-induced enzyme, relative insensitivity of pol I Tequintavirus T5

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, Mg2+ or Mn2+ Tequatrovirus T4
Mg2+ divalent cation required, Mg2+ or Mn2+ Escherichia phage T7
Mg2+ divalent cation required, Mg2+ or Mn2+ Tequintavirus T5
Mg2+ optimal concentration: 6 mM Tequatrovirus T4
Mn2+ optimal concentration: 0.1 mM Tequatrovirus T4
Mn2+ 25% of the activity with Mg2+ Tequatrovirus T4
Mn2+ divalent cation required, Mn2+ or Mg2+ Tequatrovirus T4
Mn2+ divalent cation required, Mn2+ or Mg2+ Escherichia phage T7
Mn2+ divalent cation required, Mn2+ or Mg2+ Tequintavirus T5
Na+ 0.2 M, stimulates phage T5-induced enzyme Tequintavirus T5
NH4+ 0.2 M, stimulates phage T5-induced enzyme Tequintavirus T5

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 1 * 12000 thioredoxin + 1 * 84000 Escherichia phage T7
84000
-
 1 * 12000 thioredoxin + 1 * 84000 Escherichia phage T7
96000
-
 equilibrium sedimentation Escherichia phage T7
96000
-
 equilibrium sedimentation Tequintavirus T5
114000
-
-
 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Escherichia phage T7
-
 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn Tequintavirus T5
-
 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn Tequatrovirus T4 phage T4 DNA polymerase is essential for initiation and maintenance of viral DNA replication diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia phage T7
-
 Escherichia coli infected with
-
Tequatrovirus T4
-
 Escherichia coli infected with
-
Tequintavirus T5
-
 Escherichia coli infected with
-

Purification (Commentary)

Purification (Comment) Organism
Escherichia coli infected with Tequatrovirus T4
Escherichia coli infected with Escherichia phage T7
Escherichia coli infected with Tequintavirus T5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Escherichia phage T7 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn
-
 Tequintavirus T5 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity and 5'--3' activity, phage T7-induced DNA polymerase Escherichia phage T7 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn nicked duplex is no substrate of phage T4-induced DNA polymerase Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn nicked duplex is no substrate of phage T4-induced DNA polymerase Escherichia phage T7 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn exonuclease 3'--5' and 5'--3' activity activity, phage T5-induced DNA polymerase Tequintavirus T5 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn phage T5-induced DNA polymerase Tequintavirus T5 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Escherichia phage T7 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Tequintavirus T5 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn exonuclease 3'--5', phage T4-induced DNA polymerase Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn primed single strands Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn phage T4 DNA polymerase is essential for initiation and maintenance of viral DNA replication Tequatrovirus T4 diphosphate + DNAn+1
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 12000 thioredoxin + 1 * 84000 Escherichia phage T7
monomer 1 * 114000 Tequatrovirus T4
monomer 1 * 96000 Tequintavirus T5

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 7.8
-
 Escherichia phage T7
8.5 9
-
 Tequintavirus T5

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.7 about 50% of activity maximum at pH 7.5 and pH 9.7 Tequatrovirus T4