Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiol | stimulates | Escherichia phage T7 | |
thiol | required | Tequintavirus T5 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | great sensitivity of phage-induced enzyme, relative insensitivity of pol I | Escherichia phage T7 | |
N-ethylmaleimide | great sensitivity of phage-induced enzyme, relative insensitivity of pol I | Tequatrovirus T4 | |
N-ethylmaleimide | great sensitivity of phage-induced enzyme, relative insensitivity of pol I | Tequintavirus T5 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent cation required, Mg2+ or Mn2+ | Tequatrovirus T4 | |
Mg2+ | divalent cation required, Mg2+ or Mn2+ | Escherichia phage T7 | |
Mg2+ | divalent cation required, Mg2+ or Mn2+ | Tequintavirus T5 | |
Mg2+ | optimal concentration: 6 mM | Tequatrovirus T4 | |
Mn2+ | optimal concentration: 0.1 mM | Tequatrovirus T4 | |
Mn2+ | 25% of the activity with Mg2+ | Tequatrovirus T4 | |
Mn2+ | divalent cation required, Mn2+ or Mg2+ | Tequatrovirus T4 | |
Mn2+ | divalent cation required, Mn2+ or Mg2+ | Escherichia phage T7 | |
Mn2+ | divalent cation required, Mn2+ or Mg2+ | Tequintavirus T5 | |
Na+ | 0.2 M, stimulates phage T5-induced enzyme | Tequintavirus T5 | |
NH4+ | 0.2 M, stimulates phage T5-induced enzyme | Tequintavirus T5 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
1 * 12000 thioredoxin + 1 * 84000 | Escherichia phage T7 |
84000 | - |
1 * 12000 thioredoxin + 1 * 84000 | Escherichia phage T7 |
96000 | - |
equilibrium sedimentation | Escherichia phage T7 |
96000 | - |
equilibrium sedimentation | Tequintavirus T5 |
114000 | - |
- |
Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Escherichia phage T7 | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Tequintavirus T5 | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Tequatrovirus T4 | phage T4 DNA polymerase is essential for initiation and maintenance of viral DNA replication | diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage T7 | - |
Escherichia coli infected with | - |
Tequatrovirus T4 | - |
Escherichia coli infected with | - |
Tequintavirus T5 | - |
Escherichia coli infected with | - |
Purification (Comment) | Organism |
---|---|
Escherichia coli infected with | Tequatrovirus T4 |
Escherichia coli infected with | Escherichia phage T7 |
Escherichia coli infected with | Tequintavirus T5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Escherichia phage T7 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Tequintavirus T5 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 3'--5' activity and 5'--3' activity, phage T7-induced DNA polymerase | Escherichia phage T7 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | nicked duplex is no substrate of phage T4-induced DNA polymerase | Tequatrovirus T4 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | nicked duplex is no substrate of phage T4-induced DNA polymerase | Escherichia phage T7 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 3'--5' and 5'--3' activity activity, phage T5-induced DNA polymerase | Tequintavirus T5 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | phage T5-induced DNA polymerase | Tequintavirus T5 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA | Tequatrovirus T4 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA | Escherichia phage T7 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA | Tequintavirus T5 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 3'--5', phage T4-induced DNA polymerase | Tequatrovirus T4 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | primed single strands | Tequatrovirus T4 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | phage T4 DNA polymerase is essential for initiation and maintenance of viral DNA replication | Tequatrovirus T4 | diphosphate + DNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 12000 thioredoxin + 1 * 84000 | Escherichia phage T7 |
monomer | 1 * 114000 | Tequatrovirus T4 |
monomer | 1 * 96000 | Tequintavirus T5 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 7.8 | - |
Escherichia phage T7 |
8.5 | 9 | - |
Tequintavirus T5 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.7 | about 50% of activity maximum at pH 7.5 and pH 9.7 | Tequatrovirus T4 |