BRENDA - Enzyme Database show
show all sequences of 2.7.7.69

Two genes in Arabidopsis thaliana encoding GDP-L-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability

Dowdle, J.; Ishikawa, T.; Gatzek, S.; Rolinski, S.; Smirnoff, N.; Plant J. 52, 673-689 (2007)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of isoforms Vtc2/Vtc5 double mutants. Double mutants show growth arrest immediately upon germination and the cotyledons subsequently bleach. Normal growth is restored by supplementation with ascorbate or L-galactose; construction of isoforms Vtc2/Vtc5 double mutants. Double mutants show growth arrest immediately upon germination and the cotyledons subsequently bleach. Normal growth is restored by supplementation with ascorbate or L-galactose. vtc2-1 leaves contain more mannose 6-phosphate than wild-type
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
phosphate
pH 7.5, 20°C
Arabidopsis thaliana
0.25
-
GDP-alpha-L-galactose
pH 7.5, 20°C
Arabidopsis thaliana
0.251
-
phosphate
pH 7.5, 20°C
Arabidopsis thaliana
0.667
-
GDP-alpha-L-galactose
pH 7.5, 20°C
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q8RWE8
isoform Vtc2
-
Arabidopsis thaliana
Q9FLP9
isoform Vtc5
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
VTC2 expression and GDP-L-galactose phosphorylase activity rapidly increase on transfer to high light, but the activity of other enzymes in the GDP-mannose pathway is little affected. Leaves accumulate more ascorbate after acclimatization to high light intensity
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP-alpha-D-glucose + phosphate
0.1% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-glucose 1-phosphate + ADP
-
-
-
r
ADP-alpha-D-glucose + phosphate
0.4% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-glucose 1-phosphate + ADP
-
-
-
r
alpha-L-galactose 1-phosphate + GDP
-
694667
Arabidopsis thaliana
GDP-alpha-L-galactose + phosphate
-
-
-
r
GDP-alpha-D-mannose + phosphate
0.1% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-mannose 1-phosphate + GDP
-
-
-
r
GDP-alpha-D-mannose + phosphate
3.7% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-mannose 1-phosphate + GDP
-
-
-
r
GDP-beta-L-galactose + phosphate
-
694667
Arabidopsis thaliana
GDP + beta-L-galactose 1-phosphate
-
-
-
r
UDP-alpha-D-galactose + phosphate
0.1% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-galactose 1-phosphate + UDP
-
-
-
r
UDP-alpha-D-galactose + phosphate
0.2% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-galactose 1-phosphate + UDP
-
-
-
r
UDP-alpha-D-glucose + phosphate
0.3% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-glucose 1-phosphate + UDP
-
-
-
r
UDP-alpha-D-glucose + phosphate
2.1% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-glucose 1-phosphate + UDP
-
-
-
r
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.04
-
GDP-alpha-L-galactose
pH 7.5, 20°C
Arabidopsis thaliana
2.68
-
GDP-alpha-L-galactose
pH 7.5, 20°C
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of isoforms Vtc2/Vtc5 double mutants. Double mutants show growth arrest immediately upon germination and the cotyledons subsequently bleach. Normal growth is restored by supplementation with ascorbate or L-galactose. vtc2-1 leaves contain more mannose 6-phosphate than wild-type
Arabidopsis thaliana
additional information
construction of isoforms Vtc2/Vtc5 double mutants. Double mutants show growth arrest immediately upon germination and the cotyledons subsequently bleach. Normal growth is restored by supplementation with ascorbate or L-galactose
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
phosphate
pH 7.5, 20°C
Arabidopsis thaliana
0.25
-
GDP-alpha-L-galactose
pH 7.5, 20°C
Arabidopsis thaliana
0.251
-
phosphate
pH 7.5, 20°C
Arabidopsis thaliana
0.667
-
GDP-alpha-L-galactose
pH 7.5, 20°C
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
VTC2 expression and GDP-L-galactose phosphorylase activity rapidly increase on transfer to high light, but the activity of other enzymes in the GDP-mannose pathway is little affected. Leaves accumulate more ascorbate after acclimatization to high light intensity
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP-alpha-D-glucose + phosphate
0.1% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-glucose 1-phosphate + ADP
-
-
-
r
ADP-alpha-D-glucose + phosphate
0.4% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-glucose 1-phosphate + ADP
-
-
-
r
alpha-L-galactose 1-phosphate + GDP
-
694667
Arabidopsis thaliana
GDP-alpha-L-galactose + phosphate
-
-
-
r
GDP-alpha-D-mannose + phosphate
0.1% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-mannose 1-phosphate + GDP
-
-
-
r
GDP-alpha-D-mannose + phosphate
3.7% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-mannose 1-phosphate + GDP
-
-
-
r
GDP-beta-L-galactose + phosphate
-
694667
Arabidopsis thaliana
GDP + beta-L-galactose 1-phosphate
-
-
-
r
UDP-alpha-D-galactose + phosphate
0.1% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-galactose 1-phosphate + UDP
-
-
-
r
UDP-alpha-D-galactose + phosphate
0.2% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-galactose 1-phosphate + UDP
-
-
-
r
UDP-alpha-D-glucose + phosphate
0.3% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-glucose 1-phosphate + UDP
-
-
-
r
UDP-alpha-D-glucose + phosphate
2.1% of the activity with GDP-L-galactose
694667
Arabidopsis thaliana
alpha-D-glucose 1-phosphate + UDP
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.04
-
GDP-alpha-L-galactose
pH 7.5, 20°C
Arabidopsis thaliana
2.68
-
GDP-alpha-L-galactose
pH 7.5, 20°C
Arabidopsis thaliana
Other publictions for EC 2.7.7.69
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739282
Wang
Overexpression of tomato GDP-L ...
Solanum lycopersicum
Plant Cell Rep.
33
1441-1451
2014
-
-
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4
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1
1
-
-
-
725801
Wang
Antisense-mediated depletion o ...
Solanum lycopersicum
J. Plant Physiol.
170
303-314
2013
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-
-
-
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2
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4
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2
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1
1
-
-
-
738329
Hou
Expression of a GDP-L-galactos ...
Vitis pseudoreticulata, Vitis pseudoreticulata Baihe-35-l
Genet. Mol. Res.
12
3830-3844
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
-
5
-
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5
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
739380
Li
Light and abiotic stresses reg ...
Actinidia deliciosa, Actinidia eriantha
Planta
238
535-547
2013
-
-
-
-
-
-
-
-
-
-
-
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-
5
-
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2
2
2
2
-
-
693081
Linster
A second GDP-L-galactose phosp ...
Arabidopsis thaliana
J. Biol. Chem.
283
18483-18492
2008
-
-
1
-
3
-
-
15
-
-
-
2
-
8
-
-
-
-
-
-
2
-
17
-
-
-
-
11
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
16
-
-
-
2
-
-
-
-
-
-
2
-
17
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
694703
Mueller-Moule
An expression analysis of the ...
Arabidopsis thaliana
Plant Mol. Biol.
68
31-41
2008
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
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2
-
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2
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-
-
2
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
692994
Linster
Arabidopsis VTC2 encodes a GDP ...
Arabidopsis thaliana
J. Biol. Chem.
282
18879-18885
2007
-
-
-
-
-
-
-
7
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
694667
Dowdle
Two genes in Arabidopsis thali ...
Arabidopsis thaliana
Plant J.
52
673-689
2007
-
-
-
-
1
-
-
4
-
-
-
-
-
4
-
-
-
-
-
2
-
-
12
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
12
-
-
-
-
2
-
-
-
-
-
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-
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