Literature summary for 2.7.7.65 extracted from

Oliveira, M.C.; Teixeira, R.D.; Andrade, M.O.; Pinheiro, G.M.; Ramos, C.H.; Farah, C.S.
Cooperative substrate binding by a diguanylate cyclase (2015), J. Mol. Biol., 427, 415-432.

Search for more literature for 2.7.7.65

Protein Variants

Protein Variants	Comment	Organism
K759A	mutagenesis of a conserved lysine residue, results in a severe reduction in dihuanylate cyclase activity, kcat value is almost 100fold lower than that of the wild-type protein while the K1 and K2 values do not change very much	Xanthomonas citri pv. citri

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibitory: cyclic di-GMP	Xanthomonas citri pv. citri

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.038	-	GTP	mutant K759A, Michaelis-Menten model, pH 8.0, 30°C	Xanthomonas citri pv. citri
0.061	-	GTP	Michaelis-Menten model, pH 8.0, 30°C	Xanthomonas citri pv. citri

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	or Mn2+, strictly required	Xanthomonas citri pv. citri
Mn2+	or Mg2+, strictly required	Xanthomonas citri pv. citri

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
94500	-	-	Xanthomonas citri pv. citri
360000	-	gel filtration	Xanthomonas citri pv. citri

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas citri pv. citri	Q8PPS5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 GTP	-	Xanthomonas citri pv. citri	2 diphosphate + cyclic di-3',5'-guanylate	-	?
additional information	sequential random binding model of two identical substrates binding to two equivalent GGDEF domains that come together to form a symmetrical active site	Xanthomonas citri pv. citri	?	-	?

Subunits

Subunits	Comment	Organism
More	at micromolar concentrations, the protein exists predominantly as a dimeric species	Xanthomonas citri pv. citri
multimer	x * 94500, calculated	Xanthomonas citri pv. citri

Synonyms

Synonyms	Comment	Organism
XAC0610	-	Xanthomonas citri pv. citri

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.014	-	GTP	mutant K759A, Michaelis-Menten model, pH 8.0, 30°C	Xanthomonas citri pv. citri
1.2	-	GTP	wild-type, Michaelis-Menten model, pH 8.0, 30°C	Xanthomonas citri pv. citri

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	8.5	-	Xanthomonas citri pv. citri

General Information

General Information	Comment	Organism
physiological function	protein has in vivo and in vitro diguanylate cyclase activity that leads to the production of cyclic di-GMP. Protein XAC0610 plays a role in the regulation of Xanthomonas citri motility and resistance to H2O2. XAC0610 is not subject to allosteric product inhibition. Instead, steady-state kinetics reveal a positive cooperative effect of the GTP substrate with a dissociation constant for the binding of the first GTP molecule (K1) approximately 5 times greater than the dissociation constant for the binding of the second GTP molecule (K2). The N-terminal GAF and PAS domains are required for high levels of XAC0610 diguanylate cyclase activity	Xanthomonas citri pv. citri

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Release 2023.1 (January 2023)