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Literature summary for 2.7.7.65 extracted from

  • Liu, N.; Pak, T.; Boon, E.M.
    Characterization of a diguanylate cyclase from Shewanella woodyi with cyclase and phosphodiesterase activities (2010), Mol. Biosyst., 6, 1561-1564.
    View publication on PubMed
Search for more literature for 2.7.7.65

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Shewanella woodyi

Protein Variants

Protein Variants Comment Organism
D287A/E288A mutant has no cyclase activity Shewanella woodyi
E415A mutant has cyclase activity but no phosphodiesterase activity Shewanella woodyi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Shewanella woodyi

Organism

Organism UniProt Comment Textmining
Shewanella woodyi
-
 bi-functional enzyme with cyclase and phosphodiesterase functions
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Shewanella woodyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP SwDGC has both cyclase and phosphodiesterase activities. Peaks for c-di-GMP and pGpG (5'-phosphoguanylyl-(3',5')-guanosine) appear within several min of reaction Shewanella woodyi cyclic di-3',5'-guanylate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
diguanylate cyclase
-
 Shewanella woodyi
SwDGC SwDGC has both cyclase and phosphodiesterase functions Shewanella woodyi
Swoo_2750
-
 Shewanella woodyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Shewanella woodyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Shewanella woodyi

General Information

General Information Comment Organism
physiological function SwDGC is capable of affecting extracellular polysaccharide matrix production, presumably by altering intracellular c-di-GMP levels Shewanella woodyi