Cloned (Comment) | Organism |
---|---|
recombinantly expressed in Escherichia coli. Target protein is produced mainly in the form of inclusion bodies. Both native and mutant enzymes are not inhibited by the target product during conversion of 1 mM GTP into c-di-GMP | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
R158A | mutant shows increased enzymatic activity compared to wild-type, mutant shows 4fold lower expression level compared to wild-type which might be due to higher enzymatic activity of mutant R158A and its increased toxicity for cells | Thermotoga maritima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ required, optimal activity at 2.5 mM MgCl2 | Thermotoga maritima | |
NaCl | optimal activity at 300-500 mM NaCl | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
151 | - |
pH 10, 60°C, mutant R158A | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Thermotoga maritima | cyclic di-3',5'-guanylate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DGC | - |
Thermotoga maritima |
diguanylate cyclase | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Thermotoga maritima |