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Literature summary for 2.7.7.65 extracted from
- Molecular oxygen regulates the enzymatic activity of a heme-containing diguanylate cyclase (HemDGC) for the synthesis of cyclic di-GMP (2010), Biochim. Biophys. Acta, 1804, 166-172.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| O2 | - |
Desulfotalea psychrophila |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET3a for expression in Escherichia coli BL21DE3 cells | Desulfotalea psychrophila |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
monomer | Desulfotalea psychrophila |
| 170000 | - |
determined by Blue-Native PAGE, homotetramer | Desulfotalea psychrophila |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | Desulfotalea psychrophila | - |
diphosphate + cyclic di-3',5'-guanylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfotalea psychrophila | Q6ARU5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a TALON metal affinity resin and a Superdex 200 column | Desulfotalea psychrophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | - |
Desulfotalea psychrophila | diphosphate + cyclic di-3',5'-guanylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 42000 | Desulfotalea psychrophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diguanylate cyclase | - |
Desulfotalea psychrophila |
| HemDGC | - |
Desulfotalea psychrophila |
| heme-containing diguanylate cyclase | - |
Desulfotalea psychrophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay | Desulfotalea psychrophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
activity assay | Desulfotalea psychrophila |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Desulfotalea psychrophila | |
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Release 2023.1 (January 2023)
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