Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.65 extracted from

  • Antoniani, D.; Bocci, P.; Maciag, A.; Raffaelli, N.; Landini, P.
    Monitoring of diguanylate cyclase activity and of cyclic-di-GMP biosynthesis by whole-cell assays suitable for high-throughput screening of biofilm inhibitors (2010), Appl. Microbiol. Biotechnol., 85, 1095-1104.
    View publication on PubMed
Search for more literature for 2.7.7.65

Application

Application Comment Organism
medicine diguanylate cyclases are interesting targets for new antimicrobial agents with biofilm activity Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
into the pTOPO vector Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information a mutant, carrying an adrA mutation resulting in GGDEF -> GGAAF change in AdrA protein catalytic site, is constructed Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
sulfathiazole
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Escherichia coli
-
 diphosphate + cyclic di-3',5'-guanylate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Escherichia coli diphosphate + cyclic di-3',5'-guanylate
-
 ?

Synonyms

Synonyms Comment Organism
AdrA
-
 Escherichia coli
DGC
-
 Escherichia coli
diguanylate cyclase
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function in Gram-negative bacteria production of of cyclic di-3',5'-guanylate, c-di-GMP, is the main trigger for production of extracellular polysaccharides and for biofilm formation Escherichia coli