Literature summary for 2.7.7.65 extracted from

Rao, F.; Pasunooti, S.; Ng, Y.; Zhuo, W.; Lim, L.; Liu, A.W.; Liang, Z.X.
Enzymatic synthesis of c-di-GMP using a thermophilic diguanylate cyclase (2009), Anal. Biochem., 389, 138-142.

Search for more literature for 2.7.7.65

Application

Application	Comment	Organism
synthesis	the thermophilic enzyme is a valuable tool for c-di-GMP synthesis as well as the preparation of c-di-GMP derivatives	Thermotoga maritima

Cloned(Commentary)

Cloned (Comment)	Organism
a DNA construct encoding the C-terminus of the TM1788 gene is cloned into the vector pET28b+ for expression in Escherichia col BL21DE3 cells	Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
R158A	mutation of a key residue in the putative regulatory I-site, product inhibition is substantially alleviated	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21000	-	determined by SDS-PAGE	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP	Thermotoga maritima	-	diphosphate + cyclic di-3',5'-guanylate	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X2A8	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni2+-nitrilotriacetic acid resin and a gel filtration coumn	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP	-	Thermotoga maritima	diphosphate + cyclic di-3',5'-guanylate	-	?
GTP + GTP	-	Thermotoga maritima	2 diphosphate + cyclic di-3',5'-guanylate	-	?

Subunits

Subunits	Comment	Organism
dimer	wild-type	Thermotoga maritima
monomer	R158A mutant	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
DGC	-	Thermotoga maritima
diguanylate cyclase	-	Thermotoga maritima
tDGC	-	Thermotoga maritima
thermophilic diguanylate cyclase domain protein	-	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	R158A mutant	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0433	-	GTP	R158A mutant	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	R158A mutant	Thermotoga maritima

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Release 2023.1 (January 2023)