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Literature summary for 2.7.7.64 extracted from

  • Liu, J.; Zou, Y.; Guan, W.; Zhai, Y.; Xue, M.; Jin, L.; Zhao, X.; Dong, J.; Wang, W.; Shen, J.; Wang, P.G.; Chen, M.
    Biosynthesis of nucleotide sugars by a promiscuous UDP-sugar pyrophosphorylase from Arabidopsis thaliana (AtUSP) (2013), Bioorg. Med. Chem. Lett., 23, 3764-3768.
    View publication on PubMed

Application

Application Comment Organism
synthesis use of UDP-sugar pyrophosphorylase (USP) from Arabidopsis thaliana with a galactokinase from Streptococcus pneumoniae TIGR4 (SpGalK) and an inorganic diphosphatase (PPase) to effectively synthesize UDP-sugars Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.88
-
UTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
3.74
-
dUTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
8.14
-
dTTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-azido-D-galactose 1-phosphate + UTP
-
Arabidopsis thaliana UDP-4-azido-D-galactose + diphosphate 43% conversion rate ?
6-deoxy-D-galactose 1-phosphate + UTP
-
Arabidopsis thaliana UDP-6-deoxy-D-galactose + diphosphate 29% conversion rate ?
D-galactose 1-phosphate + dTTP
-
Arabidopsis thaliana dTDP-D-galactose + diphosphate
-
?
D-galactose 1-phosphate + dUTP
-
Arabidopsis thaliana dUDP-D-galactose + diphosphate
-
?
D-galactose 1-phosphate + UTP
-
Arabidopsis thaliana UDP-D-galactose + diphosphate 95% conversion rate ?
D-glucose 1-phosphate + UTP
-
Arabidopsis thaliana UDP-D-glucose + diphosphate 28% conversion rate ?

Synonyms

Synonyms Comment Organism
USP
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27
-
dTTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
219
-
dUTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
348
-
UTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.3
-
dTTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
59
-
dUTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
121
-
UTP pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana