BRENDA - Enzyme Database show
show all sequences of 2.7.7.62

cobU-dependent assimilation of nonadenosylated cobinamide in cobA mutants of Salmonella typhimurium

O'Toole, G.A.; Escalante-Semerena, J.C.; J. Bacteriol. 175, 6328-6336 (1993)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpressed
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GTP + adenosylcobinamide phosphate
Salmonella enterica subsp. enterica serovar Typhimurium
cobalamin biosynthesis, involved in de novo synthesis of vitamin B12
diphosphate + adenosylcobinamide-GDP
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium LT2
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + adenosylcobinamide phosphate
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate + adenosylcobinamide-GDP
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium
?
GTP + adenosylcobinamide phosphate
cobalamin biosynthesis, involved in de novo synthesis of vitamin B12
651620
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate + adenosylcobinamide-GDP
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium
?
GTP + adenosylcobinamide phosphate
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium LT2
diphosphate + adenosylcobinamide-GDP
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
additional information
CobU is a bifunctional enzyme that has both kinase EC 2.7.1.156 and guanyltransferase EC 2.7.7.62 activities
651620
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium
?
additional information
CobU is a bifunctional enzyme that has both kinase EC 2.7.1.156 and guanyltransferase EC 2.7.7.62 activities
651620
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpressed
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GTP + adenosylcobinamide phosphate
Salmonella enterica subsp. enterica serovar Typhimurium
cobalamin biosynthesis, involved in de novo synthesis of vitamin B12
diphosphate + adenosylcobinamide-GDP
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + adenosylcobinamide phosphate
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate + adenosylcobinamide-GDP
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium
?
GTP + adenosylcobinamide phosphate
cobalamin biosynthesis, involved in de novo synthesis of vitamin B12
651620
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate + adenosylcobinamide-GDP
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium
?
GTP + adenosylcobinamide phosphate
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium LT2
diphosphate + adenosylcobinamide-GDP
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
additional information
CobU is a bifunctional enzyme that has both kinase EC 2.7.1.156 and guanyltransferase EC 2.7.7.62 activities
651620
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium
?
additional information
CobU is a bifunctional enzyme that has both kinase EC 2.7.1.156 and guanyltransferase EC 2.7.7.62 activities
651620
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
-
651620
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
Other publictions for EC 2.7.7.62
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739433
Singarapu
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1
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3
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1
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1
1
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1
-
-
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-
-
-
-
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-
-
-
-
724319
Newmister
Structure and Mutational Analy ...
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Biochemistry
50
5301-5313
2011
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1
1
21
-
-
-
-
-
-
2
-
8
-
-
-
-
-
-
-
-
2
2
1
-
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-
1
-
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1
-
1
21
-
-
-
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2
-
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2
2
1
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1
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-
702316
Otte
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Methanocaldococcus jannaschii
Biochemistry
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5882-5889
2009
-
-
1
-
-
-
-
2
-
-
2
1
-
4
-
-
1
-
-
-
-
-
1
1
1
-
1
2
-
-
-
-
-
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-
1
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-
-
-
-
-
2
-
-
2
1
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-
1
-
-
-
-
1
1
1
-
1
2
-
-
-
-
-
-
-
-
-
-
694244
Gray
The genome of Rhodobacter spha ...
Rhodobacter sphaeroides
Mol. Microbiol.
70
824-836
2008
-
-
1
-
-
-
-
-
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1
-
1
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1
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1
-
1
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1
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1
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1
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1
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1
-
1
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-
1
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-
-
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-
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-
674340
Otte
The thiamine kinase (YcfN) enz ...
Escherichia coli, Salmonella enterica
J. Bacteriol.
189
7310-7315
2007
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-
1
-
-
1
-
-
-
-
1
-
-
2
-
-
1
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-
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2
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1
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1
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1
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1
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-
2
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-
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-
663316
Hsu
Cloning, sequencing, expressio ...
Salmonella enterica subsp. enterica serovar Typhimurium
Protein Expr. Purif.
42
178-181
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
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1
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1
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1
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-
-
-
1
-
-
-
-
-
-
-
-
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-
644278
Warren
The biosynthesis of adenosylco ...
Bacillus megaterium, Escherichia coli, Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus DELTAH, Propionibacterium freudenreichii, Pseudomonas denitrificans (nomen rejiciendum), Salmonella enterica
Nat. Prod. Rep.
19
390-412
2002
-
-
-
1
-
-
-
-
-
-
1
7
-
19
-
-
-
-
-
-
-
-
21
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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-
-
1
7
-
-
-
-
-
-
-
-
21
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644438
Thomas
Analysis of the adenosylcobina ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Biol. Chem.
275
27576-27586
2000
-
-
-
-
4
-
-
-
-
-
-
1
-
41
-
-
1
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-
-
4
-
3
-
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-
-
-
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4
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1
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1
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4
-
3
-
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-
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-
-
-
-
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-
-
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-
644437
Thompson
Three-dimensional structure of ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
38
12995-13005
1999
-
-
-
1
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644436
Thompson
Three-dimensional structure of ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
37
7686-7695
1998
-
-
-
1
-
-
-
-
-
-
1
2
-
1
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
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-
1
2
-
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-
1
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-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644435
O'Toole
Purification and characterizat ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Biol. Chem.
270
23560-23569
1995
-
-
1
-
-
1
1
2
-
-
3
2
-
41
1
-
1
-
-
-
1
1
3
1
1
1
-
2
1
1
-
-
-
3
-
-
-
1
-
-
-
1
-
1
-
2
-
-
3
2
-
1
-
1
-
-
1
1
3
1
1
1
-
2
1
1
-
3
-
-
-
-
-
-
651620
O'Toole
cobU-dependent assimilation of ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Bacteriol.
175
6328-6336
1993
-
-
1
-
-
-
-
-
-
-
-
1
-
41
-
-
-
-
-
-
-
-
5
-
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-
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-
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1
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-
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1
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5
-
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-
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