BRENDA - Enzyme Database show
show all sequences of 2.7.7.62

Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3 resolution

Thompson, T.B.; Thomas, M.G.; Escalante-Semerena, J.C.; Rayment, I.; Biochemistry 37, 7686-7695 (1998)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
X-ray structure determined to 2.3 A resolution, crystals grown by microbatch, enzyme is crystallized in space group C222(1) with unit cell dimensions of a : 96.4 A, b : 114.4 A, and c : 106.7 A
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19770
-
3 * 19770
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GTP + adenosylcobinamide phosphate
Salmonella enterica subsp. enterica serovar Typhimurium
-
diphosphate + adenosylcobinamide-GDP
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
GTP + adenosylcobinamide phosphate
Salmonella enterica subsp. enterica serovar Typhimurium
cobalamin biosynthesis, involved in de novo synthesis of vitamin B12
diphosphate + adenosylcobinamide-GDP
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Purification (Commentary)
Commentary
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + adenosylcobinamide phosphate
-
644436
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate + adenosylcobinamide-GDP
-
644436
Salmonella enterica subsp. enterica serovar Typhimurium
?
GTP + adenosylcobinamide phosphate
cobalamin biosynthesis, involved in de novo synthesis of vitamin B12
644436
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate + adenosylcobinamide-GDP
-
644436
Salmonella enterica subsp. enterica serovar Typhimurium
?
additional information
CobU is a bifunctional enzyme that has both kinase EC 2.7.1.156 and guanyltransferase EC 2.7.7.62 activities
644436
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
644436
Salmonella enterica subsp. enterica serovar Typhimurium
?
Subunits
Subunits
Commentary
Organism
trimer
3 * 19770
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary) (protein specific)
Crystallization
Organism
X-ray structure determined to 2.3 A resolution, crystals grown by microbatch, enzyme is crystallized in space group C222(1) with unit cell dimensions of a : 96.4 A, b : 114.4 A, and c : 106.7 A
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19770
-
3 * 19770
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GTP + adenosylcobinamide phosphate
Salmonella enterica subsp. enterica serovar Typhimurium
-
diphosphate + adenosylcobinamide-GDP
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
GTP + adenosylcobinamide phosphate
Salmonella enterica subsp. enterica serovar Typhimurium
cobalamin biosynthesis, involved in de novo synthesis of vitamin B12
diphosphate + adenosylcobinamide-GDP
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + adenosylcobinamide phosphate
-
644436
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate + adenosylcobinamide-GDP
-
644436
Salmonella enterica subsp. enterica serovar Typhimurium
?
GTP + adenosylcobinamide phosphate
cobalamin biosynthesis, involved in de novo synthesis of vitamin B12
644436
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate + adenosylcobinamide-GDP
-
644436
Salmonella enterica subsp. enterica serovar Typhimurium
?
additional information
CobU is a bifunctional enzyme that has both kinase EC 2.7.1.156 and guanyltransferase EC 2.7.7.62 activities
644436
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
644436
Salmonella enterica subsp. enterica serovar Typhimurium
?
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
3 * 19770
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC 2.7.7.62
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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4
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4
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3
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644437
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38
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-
-
1
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1
1
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1
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1
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3
1
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1
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1
1
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1
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3
1
-
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644436
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Three-dimensional structure of ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
37
7686-7695
1998
-
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-
1
-
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1
2
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1
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1
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3
1
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1
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2
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1
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3
1
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644435
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Purification and characterizat ...
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J. Biol. Chem.
270
23560-23569
1995
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1
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1
1
2
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3
2
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41
1
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1
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1
1
3
1
1
1
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2
1
1
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3
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1
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1
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1
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2
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3
2
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1
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1
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1
1
3
1
1
1
-
2
1
1
-
3
-
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-
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-
-
651620
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J. Bacteriol.
175
6328-6336
1993
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1
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1
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41
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5
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1
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1
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5
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