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Literature summary for 2.7.7.62 extracted from

  • O'Toole, G.A.; Escalante-Semerena, J.C.
    Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediate (1995), J. Biol. Chem., 270, 23560-23569.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in strain JE3207 Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

General Stability Organism
substrates provides little protection from inhibition by DTNB, GTP provides slight protection from inhibition of the guanylyltransferase activity Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
DTNB
-
Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0213
-
GTP pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.102
-
5'-deoxyadenosylcobinamide phosphate pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19700
-
predicted mass, based on DNA sequence Salmonella enterica subsp. enterica serovar Typhimurium
22000
-
2 * 22000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
49000
-
gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + adenosylcobinamide phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
diphosphate + adenosylcobinamide-GDP
-
?
GTP + adenosylcobinamide phosphate Salmonella enterica subsp. enterica serovar Typhimurium assembly of the nucleotide loop of cobalamin diphosphate + adenosylcobinamide-GDP
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Oxidation Stability

Oxidation Stability Organism
in presence of oxygen guanylyltransferase activity is lost Salmonella enterica subsp. enterica serovar Typhimurium

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000202
-
-
Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
4°C, stored under a N2 headspace, retains 98% of its activity for at least 3 weeks Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + adenosylcobinamide phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + adenosylcobinamide-GDP
-
?
GTP + adenosylcobinamide phosphate assembly of the nucleotide loop of cobalamin Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + adenosylcobinamide-GDP
-
?
additional information CobU is a bifunctional enzyme that has both kinase EC 27.1.1.156 and guanyltransferase EC 2.7.7.62 activities, ATP does not serve as an efficient substrate for the CobU transferase activity, CobU does not have any detectable ATP:adenosyltransferase activity Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 22000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
bifunctional CobU enzyme
-
Salmonella enterica subsp. enterica serovar Typhimurium
CobU
-
Salmonella enterica subsp. enterica serovar Typhimurium
CobU protein
-
Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 retains 50% activity in this range, temperatures above 60°C results in drastic decrease of activity Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.058
-
GTP pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.093
-
5'-deoxyadenosylcobinamide phosphate pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8 9
-
Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9.5 50% activity retains in this range Salmonella enterica subsp. enterica serovar Typhimurium

pI Value

Organism Comment pI Value Maximum pI Value
Salmonella enterica subsp. enterica serovar Typhimurium predicted pI for the denatured protein
-
5.3
Salmonella enterica subsp. enterica serovar Typhimurium experimetally determined isoelectric point
-
7.2
Salmonella enterica subsp. enterica serovar Typhimurium experimetally determined isoelectric point
-
7.5