BRENDA - Enzyme Database
show all sequences of 2.7.7.60

Homology modeling of Mycobacterium tuberculosis 2C-methyl-D-erythritol-4-phosphate cytidylyltransferase, the third enzyme in the MEP pathway for isoprenoid biosynthesis

Obiol-Pardo, C.; Cordero, A.; Rubio-Martinez, J.; Imperial, S.; J. Mol. Model. 16, 1061-1073 (2010)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
the structure of CMS of Mycobacterium tuberculosis could be a starting point for structure-based design of new antituberculosis drugs
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.043
-
2-C-methyl-D-erythritol 4-phosphate
calculated affinity
Mycobacterium tuberculosis
0.092
-
CTP
calculated affinity
Mycobacterium tuberculosis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Mycobacterium tuberculosis
Mn2+
-
Mycobacterium tuberculosis
Zn2+
-
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + 2-C-methyl-D-erythritol 4-phosphate
Mycobacterium tuberculosis
-
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
Mycobacterium tuberculosis H37Rv
-
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
P9WKG9
-
-
Mycobacterium tuberculosis H37Rv
P9WKG9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + 2-C-methyl-D-erythritol 4-phosphate
-
705229
Mycobacterium tuberculosis
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
-
705229
Mycobacterium tuberculosis H37Rv
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
?
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
-
Mycobacterium tuberculosis
Application (protein specific)
Application
Commentary
Organism
medicine
the structure of CMS of Mycobacterium tuberculosis could be a starting point for structure-based design of new antituberculosis drugs
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.043
-
2-C-methyl-D-erythritol 4-phosphate
calculated affinity
Mycobacterium tuberculosis
0.092
-
CTP
calculated affinity
Mycobacterium tuberculosis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Mycobacterium tuberculosis
Mn2+
-
Mycobacterium tuberculosis
Zn2+
-
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CTP + 2-C-methyl-D-erythritol 4-phosphate
Mycobacterium tuberculosis
-
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
Mycobacterium tuberculosis H37Rv
-
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + 2-C-methyl-D-erythritol 4-phosphate
-
705229
Mycobacterium tuberculosis
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
-
705229
Mycobacterium tuberculosis H37Rv
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
?
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
-
Mycobacterium tuberculosis
General Information
General Information
Commentary
Organism
physiological function
2C-methyl-D-erythritol-4-phosphate cytidylyltransferase is the third enzyme in the MEP pathway for isoprenoid biosynthesis
Mycobacterium tuberculosis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
2C-methyl-D-erythritol-4-phosphate cytidylyltransferase is the third enzyme in the MEP pathway for isoprenoid biosynthesis
Mycobacterium tuberculosis
Other publictions for EC 2.7.7.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737438
Brault
Characterization of cytidylylt ...
Listeria monocytogenes
Anal. Biochem.
510
26-32
2016
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1
1
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726274
Tsang
Francisella tularensis 2-C-Met ...
Francisella tularensis, Francisella tularensis FSC155
PLoS ONE
6
e20884
2011
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1
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2
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2
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1
1
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6
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1
1
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1
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1
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1
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2
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2
1
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1
1
705229
Obiol-Pardo
Homology modeling of Mycobacte ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Model.
16
1061-1073
2010
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2
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1
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1
1
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691382
Sando
Cloning and characterization o ...
Hevea brasiliensis
Biosci. Biotechnol. Biochem.
72
2903-2917
2008
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1
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6
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692926
Shi
Biosynthesis of isoprenoids: c ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biochem. Mol. Biol.
40
911-920
2007
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1
1
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2
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5
2
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-
165
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1
1
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1
2
8
1
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2
2
2
1
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1
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2
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1
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1
2
8
1
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2
2
2
1
-
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-
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-
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-
672138
Lherbet
Absence of substrate channelin ...
Agrobacterium tumefaciens
Biochemistry
45
3548-3553
2006
-
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1
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1
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1
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1
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673537
Gabrielsen
The crystal structure of a pla ...
Arabidopsis thaliana
FEBS J.
273
1065-1073
2006
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1
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-
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5
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675583
Illarionova
Nonmevalonate terpene biosynth ...
Escherichia coli
J. Org. Chem.
71
8824-8834
2006
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1
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676369
Kim
Cloning and functional charact ...
Ginkgo biloba
Phytochemistry
67
1435-1441
2006
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1
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1
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1
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4
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1
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1
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660697
Bernal
A colorimetric assay for the d ...
Escherichia coli
Anal. Biochem.
337
55-61
2005
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1
1
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2
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1
1
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1
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1
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2
1
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656263
Gabrielsen
Hexameric assembly of the bifu ...
Agrobacterium tumefaciens, Campylobacter jejuni, Escherichia coli
J. Biol. Chem.
279
52753-52761
2004
-
-
3
3
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2
3
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6
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3
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6
2
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3
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3
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6
2
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661089
Richard
Kinetic analysis of Escherichi ...
Escherichia coli
Biochemistry
43
12189-12197
2004
-
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1
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13
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1
18
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1
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1
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1
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1
1
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2
1
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18
-
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1
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1
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13
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1
1
18
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1
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1
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2
1
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18
-
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660578
Kemp
Structure of a tetragonal crys ...
Escherichia coli
Acta Crystallogr. Sect. D
59
607-610
2003
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1
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1
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1
1
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1
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661463
Zhao
Assembly of the covalent linka ...
Escherichia coli
Chem. Biol.
10
1293-1302
2003
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1
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393239
Cane
Molecular cloning, expression ...
Escherichia coli, Streptomyces coelicolor, Streptomyces coelicolor CH999
Bioorg. Med. Chem.
9
1467-1477
2001
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1
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3
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1
3
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7
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1
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1
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1
1
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1
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3
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6
1
1
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3
1
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393240
Richard
Structure of 4-diphosphocytidy ...
Escherichia coli, Mycobacterium tuberculosis, Plasmodium falciparum
Nature Struct. Biol.
8
641-648
2001
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1
1
3
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1
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4
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7
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393241
Kemp
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. D
57
1189-1191
2001
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1
1
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2
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393242
Rohdich
Biosynthesis of terpenoids: 4- ...
Arabidopsis thaliana, Escherichia coli
Proc. Natl. Acad. Sci. USA
97
6451-6456
2000
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1
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4
2
2
3
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4
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1
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2
2
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2
2
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4
2
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2
2
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2
2
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393243
Kuzuyama
-
Formation of 4-(cytidine 5'-di ...
Escherichia coli
Tetrahedron Lett.
41
703-706
2000
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1
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1
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393244
Rohdich
Cytidine 5'-triphosphate-depen ...
Arabidopsis thaliana, Escherichia coli, Escherichia coli DH5-alpha
Proc. Natl. Acad. Sci. USA
96
11758-11763
1999
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1
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2
1
5
2
3
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16
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1
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1
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3
1
2
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3
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1
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2
1
5
2
3
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1
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3
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