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Literature summary for 2.7.7.60 extracted from

  • Cane, D.E.; Chow, C.; Lillo, A.; Kang, I.
    Molecular cloning, expression and characterization of the first three genes in the mevalonate-independent isoprenoid pathway in Streptomyces coelicolor (2001), Bioorg. Med. Chem., 9, 1467-1477.
    View publication on PubMed
Search for more literature for 2.7.7.60

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)-RP Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 2-C-methyl-D-erythritol-4-phosphate
-
 Escherichia coli
1.45
-
 CTP
-
 Streptomyces coelicolor
3.26
-
 2-C-methyl-D-erythritol-4-phosphate
-
 Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27712
-
 x * 27712, ES-MS Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 2-C-methyl-D-erythritol-4-phosphate Escherichia coli part of mevalonate-indpendent pathway for isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol-4-phosphate Streptomyces coelicolor part of mevalonate-indpendent pathway for isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol-4-phosphate Streptomyces coelicolor CH999 part of mevalonate-indpendent pathway for isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Streptomyces coelicolor
-
 CH999
-
Streptomyces coelicolor CH999
-
 CH999
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, Phenyl-Sepharose, Superdex HiLoad Streptomyces coelicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00335
-
 assay with D-1-deoxyxylulose-5-phosphate or 2-C-methyl-D-erythritol-4-phosphate Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Streptomyces coelicolor diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Streptomyces coelicolor CH999 diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol-4-phosphate part of mevalonate-indpendent pathway for isoprenoid biosynthesis Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol-4-phosphate part of mevalonate-indpendent pathway for isoprenoid biosynthesis Streptomyces coelicolor diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol-4-phosphate part of mevalonate-indpendent pathway for isoprenoid biosynthesis Streptomyces coelicolor CH999 diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Subunits

Subunits Comment Organism
? x * 27712, ES-MS Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 2-C-methyl-D-erythritol-4-phosphate
-
 Streptomyces coelicolor
6
-
 CTP
-
 Streptomyces coelicolor
16.8
-
 2-C-methyl-D-erythritol-4-phosphate
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
 Streptomyces coelicolor