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Literature summary for 2.7.7.6 extracted from

  • Buck, S.W.; Maqani, N.; Matecic, M.; Hontz, R.D.; Fine, R.D.; Li, M.; Smith, J.S.
    RNA polymerase I and Fob1 contributions to transcriptional silencing at the yeast rDNA locus (2016), Nucleic Acids Res., 44, 6173-6184.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
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Saccharomyces cerevisiae 5634
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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derivative of strain GRF167
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Saccharomyces cerevisiae JB740
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derivative of strain GRF167
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information quantitative reverse transcriptase-PCR expression analysis Saccharomyces cerevisiae ?
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?
additional information quantitative reverse transcriptase-PCR expression analysis Saccharomyces cerevisiae JB740 ?
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?

Synonyms

Synonyms Comment Organism
RNA polymerase I
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Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction rDNA silencing is attenuated by loss of Pol I subunits or insertion of an ectopic Pol I terminator within the adjacent rDNA gene. Silencing left of the rDNA array is naturally attenuated by the presence of only one intact Fob1 binding site (Ter2). Repair of the 2nd Fob1 binding site (Ter1) dramatically strengthens silencing such that it is no longer impacted by local Pol I transcription defects. Global loss of Pol I activity, negatively affects Fob1 association with the rDNA. Loss of Ter2 almost completely eliminates localized silencing, but is restored by artificially targeting Fob1 or Sir2 as Gal4 DNA binding domain fusions Saccharomyces cerevisiae
physiological function active Pol I transcription is critical for silencing of Pol II transcription within the rDNA. Sir2 is recruited to the rDNA promoter through interactions with RNA polymerase I, Sir2 suppresses RNA polymerase II (Pol II)-transcribed genes embedded within the yeast rDNA locus, i.e. rDNA silencing. Fob1 and Pol Imake independent contributions to establishment of silencing, though Pol I also reinforces Fob1-dependent silencing Saccharomyces cerevisiae