Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by 0.1 mg/ml of rifampicin, streptolydigin or alpha-amanitin | Desulfurococcus mucosus | |
additional information | no inhibition by 0.1 mg/ml of rifampicin, streptolydigin or alpha-amanitin | Sulfolobus acidocaldarius | |
additional information | no inhibition by 0.1 mg/ml of rifampicin, streptolydigin or alpha-amanitin | Thermoproteus tenax | |
oxygen | the enzyme is highly oxygen sensitive. Inactivation is accompanied by cross-linking of components. Inactivated enzyme can be reactivated by reduction with sodium dithionite | Thermoproteus tenax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | optimal activity at 0.02 M MgCl2 and 0.1 M KCl | Sulfolobus acidocaldarius | |
K+ | optimal activity at 0.02 M MgCl2 and 0.1 M KCl | Thermoproteus tenax | |
K+ | optimal activity at 0.02 M MgCl2 and 0.1 M KCl | Desulfurococcus mucosus | |
Mg2+ | optimal activity at 0.02 M MgCl2 and 0.1 M KCl | Sulfolobus acidocaldarius | |
Mg2+ | optimal activity at 0.02 M MgCl2 and 0.1 M KCl | Thermoproteus tenax | |
Mg2+ | optimal activity at 0.02 M MgCl2 and 0.1 M KCl | Desulfurococcus mucosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfurococcus mucosus | - |
- |
- |
Desulfurococcus mucosus 07 | - |
- |
- |
Sulfolobus acidocaldarius | - |
- |
- |
Sulfolobus acidocaldarius DSM 639 | - |
- |
- |
Thermoproteus tenax | - |
- |
- |
Thermoproteus tenax DSM 2078 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is highly oxygen sensitive. Inactivation is accompanied by cross-linking of components. Inactivated enzyme can be reactivated by reduction with sodium dithionite | Thermoproteus tenax |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
- |
Thermoproteus tenax |
- |
Desulfurococcus mucosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | DNA-template dependent reaction. T7 DNA and the plasmid PBR322 are by far the best templates. P2, T4 and T5 DNA are weak templates | Desulfurococcus mucosus | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | DNA-template dependent reaction. The best of the templates is phiH DNA, whereas T7 and T4 DNA are comparatively inactive and P2 DNA is a very weak template | Sulfolobus acidocaldarius | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | DNA-template dependent reaction. The enzyme transcribes phiH DNA as efficiently and T4 DNA as weakly as the Sulfolobus enzyme but T7 DNA even better than phiH DNA | Thermoproteus tenax | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | DNA-template dependent reaction. The best of the templates is phiH DNA, whereas T7 and T4 DNA are comparatively inactive and P2 DNA is a very weak template | Sulfolobus acidocaldarius DSM 639 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | DNA-template dependent reaction. The enzyme transcribes phiH DNA as efficiently and T4 DNA as weakly as the Sulfolobus enzyme but T7 DNA even better than phiH DNA | Thermoproteus tenax DSM 2078 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | DNA-template dependent reaction. T7 DNA and the plasmid PBR322 are by far the best templates. P2, T4 and T5 DNA are weak templates | Desulfurococcus mucosus 07 | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | components of the RNA polymerase and their molecular weights determined by SDS-PAGE | Sulfolobus acidocaldarius |
More | components of the RNA polymerase and their molecular weights determined by SDS-PAGE | Thermoproteus tenax |
More | components of the RNA polymerase and their molecular weights determined by SDS-PAGE | Desulfurococcus mucosus |
Synonyms | Comment | Organism |
---|---|---|
DNA-dependent RNA polymerase | - |
Sulfolobus acidocaldarius |
DNA-dependent RNA polymerase | - |
Thermoproteus tenax |
DNA-dependent RNA polymerase | - |
Desulfurococcus mucosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
assay at | Sulfolobus acidocaldarius |
85 | - |
assay at | Thermoproteus tenax |
85 | - |
assay at | Desulfurococcus mucosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sulfolobus acidocaldarius |
8 | - |
assay at | Thermoproteus tenax |
8 | - |
assay at | Desulfurococcus mucosus |
8 | 8.5 | - |
Sulfolobus acidocaldarius |
8 | 8.5 | - |
Thermoproteus tenax |
8 | 8.5 | - |
Desulfurococcus mucosus |