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Literature summary for 2.7.7.6 extracted from
- Remodeling and activation of Escherichia coli RNA polymerase by osmolytes (2008), Biochemistry, 47, 13189-13196.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutamate | glutamate remodels the sigma38 transcription complex for activation. Accumulation of the simple signaling molecule glutamate can reprogram RNA polymerase in vitro without the need for specific protein receptors. During osmotic activation, glutamate appears to act as a Hofmeister series osmolyte to facilitate promoter escape. Escape is accompanied by a remodeling of the key interaction between the sigma38 stress protein and the beta-flap of the bacterial core RNA polymerase. This activation event contrasts with the established mechanism of inhibition in which glutamate, by virtue of its electrostatic properties, helps to inhibit binding to ribosomal promoters after osmotic shock | Escherichia coli |  |
| potassium acetate | activates | Escherichia coli | |
| potassium aspartate | activates | Escherichia coli | |
| potassium chloride | activates | Escherichia coli | |
| potassium glutamate | activates highly, role of potassium ion in the activation of osmotic transcription | Escherichia coli | |
| potassium nitrate | activates | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | Escherichia coli | regulation by anions, overview | diphosphate + RNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | regulation by anions, overview | Escherichia coli | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | template is supercoiled DNA | Escherichia coli | diphosphate + RNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNA polymerase | - |
Escherichia coli |
| sigma38 RNA polymerase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Escherichia coli |
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