Literature summary for 2.7.7.6 extracted from

Brueckner, F.; Cramer, P.
Structural basis of transcription inhibition by alpha-amanitin and implications for RNA polymerase II translocation (2008), Nat. Struct. Mol. Biol., 15, 811-818.

Search for more literature for 2.7.7.6

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with alpha-amanitin, hanging drop vapour diffusion method, with 200 mM ammonium acetate, 300 mM sodium acetate, 50 mM HEPES, pH 7.0, 4-7% (w/v) PEG 6000 and 5 mM Tris(2-carboxyethyl) phosphine	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
alpha-Amanitin	alpha-amanitin inhibits Pol II by trapping the wedged trigger loop and shifted bridge helix, thereby stabilizing a conformation of the elongation complex that apparently represents a translocation intermediate	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
chromatography	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nucleoside triphosphate + RNAn	-	Saccharomyces cerevisiae	diphosphate + RNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
Pol II	-	Saccharomyces cerevisiae
RNA polymerase II	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)