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Literature summary for 2.7.7.6 extracted from

  • Zillig, W.; Stetter, K.O.; Janekovic, D.
    DNA-dependent RNA polymerase from the archaebacterium Sulfolobus acidocaldarius (1979), Eur. J. Biochem., 96, 597-604.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in a phage display using Halobacterium phage phi H, method overview Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10800
-
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
11200
-
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
11800
-
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
13800
-
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
17500
-
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
24000
-
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
32000
-
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
44000
-
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
101000
-
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
122000
-
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
423500
-
-
Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
strain DSM 639
-

Purification (Commentary)

Purification (Comment) Organism
polyethylene imine precipitation, anion exchange chromatography, heparin and T4-DNA affinity chronatography, and sucrose gradient centrifugation Sulfolobus acidocaldarius

Storage Stability

Storage Stability Organism
the purified enzyme activity is stable in solution at 4°C even at low ionic strength and in the absence of bivalent metal ions or glycerol, storage in a buffer of low ionic strength containing no bivalent metal ions but 0.001 M EDTA and 10% glycerol at -70°C Sulfolobus acidocaldarius

Subunits

Subunits Comment Organism
decamer 1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE Sulfolobus acidocaldarius
More at 0.5 M NH4CI the enzyme exists in the monomeric form Sulfolobus acidocaldarius

Synonyms

Synonyms Comment Organism
DNA-dependent RNA polymerase
-
Sulfolobus acidocaldarius