Cloned (Comment) | Organism |
---|---|
expression in a phage display using Halobacterium phage phi H, method overview | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10800 | - |
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
11200 | - |
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
11800 | - |
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
13800 | - |
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
17500 | - |
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
24000 | - |
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
32000 | - |
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
44000 | - |
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
101000 | - |
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
122000 | - |
1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
423500 | - |
- |
Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | - |
strain DSM 639 | - |
Purification (Comment) | Organism |
---|---|
polyethylene imine precipitation, anion exchange chromatography, heparin and T4-DNA affinity chronatography, and sucrose gradient centrifugation | Sulfolobus acidocaldarius |
Storage Stability | Organism |
---|---|
the purified enzyme activity is stable in solution at 4°C even at low ionic strength and in the absence of bivalent metal ions or glycerol, storage in a buffer of low ionic strength containing no bivalent metal ions but 0.001 M EDTA and 10% glycerol at -70°C | Sulfolobus acidocaldarius |
Subunits | Comment | Organism |
---|---|---|
decamer | 1 * 122000 + 1 * 101000 + 1 * 44000 + 1 * 32000 + 1 * 24000 + 1 * 17500 + 1 * 13800 + 4 * 11800 + 1 * 11200 + 1 * 10800, SDS-PAGE | Sulfolobus acidocaldarius |
More | at 0.5 M NH4CI the enzyme exists in the monomeric form | Sulfolobus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
DNA-dependent RNA polymerase | - |
Sulfolobus acidocaldarius |