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Literature summary for 2.7.7.6 extracted from
- T7 RNA polymerase mediates fast promoter-independent extension of unstable nucleic acid complexes (2004), Biochemistry, 43, 7873-7880.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000016 | - |
promoter complex | - |
Escherichia phage T7 | |
| 0.0018 | - |
T10G2T2C2C | - |
Escherichia phage T7 | |
| 0.018 | - |
A10G2A2C2C | - |
Escherichia phage T7 | |
| 0.041 | - |
A9G3A2C2C | - |
Escherichia phage T7 | |
| 0.043 | - |
G2CAC2C | - |
Escherichia phage T7 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | Escherichia phage T7 | mediates fast promoter-independent extension of unstable nucleic acid complexes | diphosphate + RNAn+1 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia phage T7 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + A10G2A2C2C | oligonucleotide extension | Escherichia phage T7 | ? | - |
? | |
| nucleoside triphosphate + A9G3A2C2C | oligonucleotide extension | Escherichia phage T7 | ? | - |
? | |
| nucleoside triphosphate + G2CAC2C | oligonucleotide extension | Escherichia phage T7 | ? | - |
? | |
| nucleoside triphosphate + promoter complex | - |
Escherichia phage T7 | ? | - |
? | |
| nucleoside triphosphate + RNAn | mediates fast promoter-independent extension of unstable nucleic acid complexes | Escherichia phage T7 | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | short DNA or RNA substrates are good substrates for the enzyme | Escherichia phage T7 | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + T10G2T2C2C | oligonucleotide extension | Escherichia phage T7 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNAP | - |
Escherichia phage T7 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
G2CAC2C | - |
Escherichia phage T7 | |
| 0.23 | - |
T10G2T2C2C | - |
Escherichia phage T7 | |
| 0.26 | - |
A10G2A2C2C | - |
Escherichia phage T7 | |
| 4 | - |
A9G3A2C2C | - |
Escherichia phage T7 | |
| 8.3 | - |
promoter complex | - |
Escherichia phage T7 |
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