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Literature summary for 2.7.7.6 extracted from
- DNA-dependent RNA polymerase from Pseudomonas aeruginosa (1987), Biochem. Cell Biol., 65, 776-782.
General Stability
| General Stability | Organism |
|---|---|
| each freeze-thaw cycle results in significant loss of activity | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| actinomycin D | - |
Pseudomonas aeruginosa |  |
| heparin | - |
Pseudomonas aeruginosa | |
| KCl | above 10 mM | Pseudomonas aeruginosa | |
| rifampicin | - |
Pseudomonas aeruginosa | |
| Streptolydigin | - |
Pseudomonas aeruginosa | |
| streptovaracin | - |
Pseudomonas aeruginosa |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, optimal activity at 10 mM MgCl2 | Pseudomonas aeruginosa | |
| Mn2+ | can partially replace Mg2+, 20% of the activity with Mg2+ | Pseudomonas aeruginosa | |
| additional information | KCl, ZnCl2 and CaCl2 can not replace MgCl2 in the assay mixture | Pseudomonas aeruginosa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
beta,beta,sigma,alpha. Subunit stoichiometry is 1:1:1:2, x * 157000 + x * 148000 + x * 87000 + 2 * x * 45000, SDS-PAGE | Pseudomonas aeruginosa |
| 87000 | - |
beta,beta,sigma,alpha. Subunit stoichiometry is 1:1:1:2, x * 157000 + x * 148000 + x * 87000 + 2 * x * 45000, SDS-PAGE | Pseudomonas aeruginosa |
| 148000 | - |
beta,beta,sigma,alpha. Subunit stoichiometry is 1:1:1:2, x * 157000 + x * 148000 + x * 87000 + 2 * x * 45000, SDS-PAGE | Pseudomonas aeruginosa |
| 157000 | - |
beta,beta,sigma,alpha. Subunit stoichiometry is 1:1:1:2, x * 157000 + x * 148000 + x * 87000 + 2 * x * 45000, SDS-PAGE | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000178 | - |
- |
Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, -20°C or -70°C, 50% w/v glycerol, stable for more than 3 months | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | the enzyme is able to use a variety of DNA templates. DNA from bacteriphage phiPLS27 is transcribed more efficiently than DNA isolated from lamda or herring sperm. DNA isolated from bacteriophage T7 and T7 D111 is utilized more efficiently | Pseudomonas aeruginosa | diphosphate + RNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | beta',beta,sigma,alpha. Subunit stoichiometry is 1:1:1:2, x * 157000 + x * 148000 + x * 87000 + 2 * x * 45000, SDS-PAGE | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
Tris-HCl buffer | Pseudomonas aeruginosa |
| 9 | - |
glycine-NaOH buffer | Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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