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Literature summary for 2.7.7.6 extracted from
- Purification and partial characterization of the DNA-dependent RNA polymerase from Rickettsia prowazekii (1990), J. Bacteriol., 172, 5624-5630.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | 74% of the RNA polymerase activity is associated with the rickettsial cell membrane at a high salt concentration, 600 mM NaCl | Rickettsia prowazekii | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required. Optimal activity at 10 mM MgCl2 and 50 mM NaCl | Rickettsia prowazekii |  |
| NaCl | optimal activity at 50 mM NaCl2 | Rickettsia prowazekii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34500 | - |
betax,betax,sigmax,alphax, x * 148000 + x * 142000 + x * 85000 + x * 34500, SDS-PAGE | Rickettsia prowazekii |
| 85000 | - |
betax,betax,sigmax,alphax, x * 148000 + x * 142000 + x * 85000 + x * 34500, SDS-PAGE | Rickettsia prowazekii |
| 142000 | - |
betax,betax,sigmax,alphax, x * 148000 + x * 142000 + x * 85000 + x * 34500, SDS-PAGE | Rickettsia prowazekii |
| 148000 | - |
betax,betax,sigmax,alphax, x * 148000 + x * 142000 + x * 85000 + x * 34500, SDS-PAGE | Rickettsia prowazekii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rickettsia prowazekii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rickettsia prowazekii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0168 | - |
- |
Rickettsia prowazekii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Rickettsia prowazekii | diphosphate + RNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | betax,betax,sigmax,alphax, x * 148000 + x * 142000 + x * 85000 + x * 34500, SDS-PAGE | Rickettsia prowazekii |
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Release 2023.1 (January 2023)
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