Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | required compound, uridylylation assay with 5 mM 2-oxoglutarate | Herbaspirillum seropedicae | |
ATP | required compound, uridylylation assay with 0.2 mM ATP | Herbaspirillum seropedicae |
Cloned (Comment) | Organism |
---|---|
expression in Echerichia coli RB9065lambdaDE3 | Herbaspirillum seropedicae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutamine | shows an inhibition of 65% of GlnB uridylylation and 70% of GlnK uridylylation | Herbaspirillum seropedicae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | uridylylation assay with 100 mM KCl | Herbaspirillum seropedicae | |
Mg2+ | uridylylation assay with 25 mM Mg2+ | Herbaspirillum seropedicae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Herbaspirillum seropedicae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GlnD is less soluble than the purified PII proteins GlnB or GlnK, to improve solubility of GlnD a special buffer is utilised (50 mM Tris-HCl, pH 7.0, 200 mM NaCl, 1 mM DTT) which produces 50% solubilization | Herbaspirillum seropedicae |
protein is purified by anionic exchange chromatography (DEAE-Sepharose column) and gel chromatography (Agarose-Heparin column) | Herbaspirillum seropedicae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + [protein-PII] | GlnD of Herbaspirillum seropedicae is overexpressed with the two PII proteins GlnK and GlnB. Results show that GlnD uridylylates GlnB and GlnK trimers producing the forms PII(UMP)1, PII(UMP)2 and PII(UMP)3. GlnB is more efficiently uridylylated than GlnK | Herbaspirillum seropedicae | diphosphate + uridylyl-[protein-PII] | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GlnD | - |
Herbaspirillum seropedicae |
uridylyltransferase | - |
Herbaspirillum seropedicae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Herbaspirillum seropedicae |