Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli, recombination of mutant gene into genomic glnD region via double crossover, mutnat VF39-BB3 and VF39-BB1 | Rhizobium leguminosarum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant construction by transposon Tn5 insertion and by subcloning + double crossover for recombination, phenotype characterization. Mutants are unable to utilize nitrate, essential function of glnD since most mutations close to the 5'-end are lethal | Rhizobium leguminosarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + [protein-PII] | Rhizobium leguminosarum | enzyme is not essential for nitrogen fixation of the host plant | diphosphate + uridylyl-[protein-PII] | - |
? | |
UTP + [protein-PII] | Rhizobium leguminosarum | the enzyme acts as the primary nitrogen sensor in the nitrogen regulation system | diphosphate + uridylyl-[protein-PII] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium leguminosarum | Q9RAE4 | from root nodules of Vicia hirsuta | - |
Rhizobium leguminosarum | Q9RAE4 | bv. viciae, constitutive gene glnD | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root nodule | enzyme expression | Rhizobium leguminosarum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + [protein-PII] | - |
Rhizobium leguminosarum | diphosphate + uridylyl-[protein-PII] | - |
? | |
UTP + [protein-PII] | enzyme is not essential for nitrogen fixation of the host plant | Rhizobium leguminosarum | diphosphate + uridylyl-[protein-PII] | - |
? | |
UTP + [protein-PII] | the enzyme acts as the primary nitrogen sensor in the nitrogen regulation system | Rhizobium leguminosarum | diphosphate + uridylyl-[protein-PII] | - |
? |