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Literature summary for 2.7.7.56 extracted from

  • Bralley, P.; Gust, B.; Chang, S.; Chater, K.F.; Jones, G.H.
    RNA 3-tail synthesis in Streptomyces: in vitro and in vivo activities of RNase PH, the SCO3896 gene product and polynucleotide phosphorylase (2006), Microbiology, 152, 627-636.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3)pLysS Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26400
-
SDS-PAGE Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor M145
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon column chromatography and Source Q column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNAn+1 + phosphate
-
Streptomyces coelicolor tRNAn + a nucleoside diphosphate
-
?
tRNAn+1 + phosphate
-
Streptomyces coelicolor M145 tRNAn + a nucleoside diphosphate
-
?

Synonyms

Synonyms Comment Organism
RNase PH
-
Streptomyces coelicolor