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Literature summary for 2.7.7.56 extracted from
- 3 Processing of tRNA precursors in ribonuclease-deficient Escherichia coli. Development and characterization of an in vitro processing system and evidence for a phosphate requirement (1988), J. Biol. Chem., 263, 1518-1523.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | enzyme works optimally at 50 mM KCl, inhibition at 200 mM | Escherichia coli |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | no effect | Escherichia coli | |
| KCl | enzyme works optimally at 50 mM KCl, inhibition at 200 mM | Escherichia coli | |
| Mg2+ | required | Escherichia coli | |
| Mg2+ | optimal activity at 3 mM | Escherichia coli | |
| Mn2+ | no effect | Escherichia coli | |
| additional information | no effect: Cd2+ | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tRNAn+1 + phosphate | Escherichia coli | brings about the final exonucleolytic trimming of the 3'-terminus of tRNA precursors in E. coli by phosphorolysis, producing a mature 3'-terminus on tRNA and nucleoside diphosphate | tRNA + a nucleoside diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
strain deficient in 5 ribonucleases | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(A) + phosphate | - |
Escherichia coli | ? | - |
r | |
| tRNAn+1 + phosphate | - |
Escherichia coli | tRNA + a nucleoside diphosphate | - |
r | |
| tRNAn+1 + phosphate | brings about the final exonucleolytic trimming of the 3'-terminus of tRNA precursors in E. coli by phosphorolysis, producing a mature 3'-terminus on tRNA and nucleoside diphosphate | Escherichia coli | tRNA + a nucleoside diphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Escherichia coli |
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