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Literature summary for 2.7.7.53 extracted from

  • Mori, S.; Shibayama, K.; Wachino, J.; Arakawa, Y.
    Structural insights into the novel diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2011), J. Mol. Biol., 410, 93-104.
    View publication on PubMed
Search for more literature for 2.7.7.53

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure at 1.89 A resolution by using single-wavelength anomalous dispersion. Asn139, Gly146, and Ser147 are important active-site residues, Asn139 has a critical role in catalysis. The position of Gly146 might influence the phosphorylase activity. The tetrameric structure and the presence of Trp160 might be essential for the formation of the Ap4A binding site Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
G146Q about 20% of wild-type activity Mycobacterium tuberculosis
G146S about 30% of wild-type activity Mycobacterium tuberculosis
N139A complete loss of activity Mycobacterium tuberculosis
N139Q complete loss of activity Mycobacterium tuberculosis
N139Q/S147T complete loss of activity Mycobacterium tuberculosis
S147A about 8% of wild-type activity Mycobacterium tuberculosis
S147T about 15% of wild-type activity Mycobacterium tuberculosis
W160A complete loss of activity Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant S147A, pH 7.6, 37°C Mycobacterium tuberculosis
0.08
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant G146Q, pH 7.6, 37°C Mycobacterium tuberculosis
0.08
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant G146S, pH 7.6, 37°C Mycobacterium tuberculosis
0.1
-
 P1,P4-bis(5'-adenosyl) tetraphosphate wild-type, pH 7.6, 37°C Mycobacterium tuberculosis
0.11
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant S147T, pH 7.6, 37°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WMK9
-
-
Mycobacterium tuberculosis H37Rv P9WMK9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Mycobacterium tuberculosis ADP + ATP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Mycobacterium tuberculosis H37Rv ADP + ATP
-
 ?

Synonyms

Synonyms Comment Organism
5',5'''-P1,P4-tetraphosphate phosphorylase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant S147A, pH 7.6, 37°C Mycobacterium tuberculosis
1.38
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant G146Q, pH 7.6, 37°C Mycobacterium tuberculosis
1.49
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant S147T, pH 7.6, 37°C Mycobacterium tuberculosis
1.99
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant G146S, pH 7.6, 37°C Mycobacterium tuberculosis
8.48
-
 P1,P4-bis(5'-adenosyl) tetraphosphate wild-type, pH 7.6, 37°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.9
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant S147A, pH 7.6, 37°C Mycobacterium tuberculosis
13.6
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant S147T, pH 7.6, 37°C Mycobacterium tuberculosis
17.25
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant G146Q, pH 7.6, 37°C Mycobacterium tuberculosis
24.88
-
 P1,P4-bis(5'-adenosyl) tetraphosphate mutant G146S, pH 7.6, 37°C Mycobacterium tuberculosis
84.8
-
 P1,P4-bis(5'-adenosyl) tetraphosphate wild-type, pH 7.6, 37°C Mycobacterium tuberculosis