Cloned (Comment) | Organism |
---|---|
DNA sequence determination, gene is located on chromosome 9, expression as His-tagged protein in Escherichia coli | Leishmania tarentolae |
DNA sequence determination, mitochondrial enzyme, expression as His-tagged protein in Escherichia coli | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
brRNA | inhibits the number of added residues | Leishmania tarentolae | |
additional information | RNA interference, i.e. RNAi, induced by tetracyclines causes down-regulation of RET1 and RET2 expression in vitro and in vivo | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Trypanosoma brucei | 5739 | - |
mitochondrion | - |
Leishmania tarentolae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Leishmania tarentolae | |
Mn2+ | requires Mn2+ or Mg2+ | Leishmania tarentolae | |
Mn2+ | selectively inhibits the distributive activity of the enzyme | Leishmania tarentolae | |
Mn2+ | when GTP, CTP, or ATP are used as substrates, Mn2+ is more effective than Mg2+ | Leishmania tarentolae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
135000 | - |
native PAGE | Leishmania tarentolae |
500000 | - |
native PAGE and gel filtration | Trypanosoma brucei |
700000 | - |
RNA-stabilized higher complexed form, native PAGE and glycerol gradient sedimentation | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma brucei | - |
? | - |
? | |
additional information | Leishmania tarentolae | enzyme interacts with a minor fraction of total RNA ligase | ? | - |
? | |
UTP + RNAn | Trypanosoma brucei | - |
diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania tarentolae | - |
- |
- |
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.67 | - |
purified enzyme | Leishmania tarentolae |
8.2 | - |
purified recombinant enzyme | Leishmania tarentolae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNAn | low activity | Leishmania tarentolae | diphosphate + RNAn+1 | - |
? | |
CTP + RNAn | - |
Leishmania tarentolae | diphosphate + RNAn+1 | - |
? | |
GTP + RNAn | about 50% of the activity with UTP, prefers Mn2+ as divalent cation | Leishmania tarentolae | diphosphate + RNAn+1 | - |
? | |
additional information | - |
Trypanosoma brucei | ? | - |
? | |
additional information | enzyme interacts with a minor fraction of total RNA ligase | Leishmania tarentolae | ? | - |
? | |
UTP + RNAn | - |
Trypanosoma brucei | diphosphate + RNAn+1 | - |
? | |
UTP + RNAn | RNA substrate specificity, overview | Leishmania tarentolae | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme is a member of the DNA polymerase beta nucleotidyltransferase superfamily | Trypanosoma brucei |
More | enzyme is a member of the DNA polymerase beta nucleotidyltransferase superfamily | Leishmania tarentolae |