Literature summary for 2.7.7.50 extracted from

Doamekpor, S.K.; Sanchez, A.M.; Schwer, B.; Shuman, S.; Lima, C.D.
How an mRNA capping enzyme reads distinct RNA polymerase II and Spt5 CTD phosphorylation codes (2014), Genes Dev., 28, 1323-1336.

Crystallization (Commentary)

Crystallization (Comment)	Organism
GTase in complex with C-terminal domain repeats of RNA polymerase Pol2 reveals a unique docking site on the nucleotidyl transferase domain for an 8-amino-acid Pol2 C-terminal domain segment, S5PPSYSPTS5P, bracketed by two Ser5-PO4 marks. At least one of the two Ser5-PO4-binding sites is required for cell viability and each site is important for cell growth at 37°C. GTase binds the Spt5 C-terminal domain at a separate docking site in the OB-fold domain that captures the Trp4 residue of the Spt5 nonapeptide repeat T1PAW4NSGSK	Schizosaccharomyces pombe

Crystallization (Comment)

Organism

GTase in complex with C-terminal domain repeats of RNA polymerase Pol2 reveals a unique docking site on the nucleotidyl transferase domain for an 8-amino-acid Pol2 C-terminal domain segment, S5PPSYSPTS5P, bracketed by two Ser5-PO4 marks. At least one of the two Ser5-PO4-binding sites is required for cell viability and each site is important for cell growth at 37°C. GTase binds the Spt5 C-terminal domain at a separate docking site in the OB-fold domain that captures the Trp4 residue of the Spt5 nonapeptide repeat T1PAW4NSGSK

Schizosaccharomyces pombe

Protein Variants

Protein Variants	Comment	Organism
G164T	56ld increase in Kd value for Pol2 CTDSer5-PO4	Schizosaccharomyces pombe
G164T/H201N	349foldd increase in Kd value for Pol2 CTDSer5-PO4	Schizosaccharomyces pombe
H201N	46ld increase in Kd value for Pol2 CTDSer5-PO4	Schizosaccharomyces pombe
H201N/R364A/Y368F	98fold increase in Kd value for Pol2 CTDSer5-PO4	Schizosaccharomyces pombe
R157E	85fold increase in Kd value for Pol2 CTDSer5-PO4	Schizosaccharomyces pombe
R157E/H201N	526fold increase in Kd value for Pol2 CTDSer5-PO4	Schizosaccharomyces pombe

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	P40997	mRNA-capping enzyme subunit alpha	-
Schizosaccharomyces pombe ATCC 24843	P40997	mRNA-capping enzyme subunit alpha	-

Synonyms

Synonyms	Comment	Organism
Ceg1	-	Schizosaccharomyces pombe
GTase	-	Schizosaccharomyces pombe

General Information

General Information	Comment	Organism
physiological function	a disruptive mutation in the polypeptide Spt5 C-terminal domain-binding site of GTase is synthetically lethal with mutations in the polymerase Pol2 C-terminal domain-binding site. C-terminal domain phosphorylation has opposite effects on the interaction of GTase with Pol2 (Ser5-PO4 is required for binding) versus Spt5 (Thr1-PO4 inhibits binding)	Schizosaccharomyces pombe