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Literature summary for 2.7.7.50 extracted from

  • Tiggemann, M.; Jeske, S.; Larsen, M.; Meinhardt, F.
    Kluyveromyces lactis cytoplasmic plasmid pGKL2: heterologous expression of Orf3p and proof of guanylyltransferase and mRNA-triphosphatase activities (2001), Yeast, 18, 815-825.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant K177A in Bacillus megaterium as His-tagged proteins Kluyveromyces lactis

Protein Variants

Protein Variants Comment Organism
K177A site-directed mutagenesis, no activity Kluyveromyces lactis

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Kluyveromyces lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
x * 67000, covalent enzyme-GMP complex, SDS-PAGE Kluyveromyces lactis

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis
-
Orf3p gene product
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant K177A protein from Bacillus megaterium Kluyveromyces lactis

Reaction

Reaction Comment Organism Reaction ID
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA conserved KXDG-motif Kluyveromyces lactis
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA active site Lys177 Kluyveromyces lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA specific for GTP Kluyveromyces lactis G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?
additional information no activity with ATP Kluyveromyces lactis ?
-
?

Subunits

Subunits Comment Organism
? x * 67000, covalent enzyme-GMP complex, SDS-PAGE Kluyveromyces lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Kluyveromyces lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Kluyveromyces lactis