Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant K177A in Bacillus megaterium as His-tagged proteins | Kluyveromyces lactis |
Protein Variants | Comment | Organism |
---|---|---|
K177A | site-directed mutagenesis, no activity | Kluyveromyces lactis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Kluyveromyces lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, covalent enzyme-GMP complex, SDS-PAGE | Kluyveromyces lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces lactis | - |
Orf3p gene product | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant K177A protein from Bacillus megaterium | Kluyveromyces lactis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | conserved KXDG-motif | Kluyveromyces lactis | |
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | active site Lys177 | Kluyveromyces lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')RNA | specific for GTP | Kluyveromyces lactis | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? | |
additional information | no activity with ATP | Kluyveromyces lactis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, covalent enzyme-GMP complex, SDS-PAGE | Kluyveromyces lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Kluyveromyces lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Kluyveromyces lactis |