Literature summary for 2.7.7.50 extracted from

Martinez-Costas, J.; Sutton, G.; Ramadevi, N.; Roy, P.
Guanylyltransferase and RNA 5'-triphosphatase activities of the purified expressed VP4 protein of bluetongue virus (1998), J. Mol. Biol., 280, 859-866.

Search for more literature for 2.7.7.50

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Spodoptera frugiperda cells via infection with baculovirus	bluetongue virus serotype 10

Organism

Organism	UniProt	Comment	Textmining
bluetongue virus serotype 10	-	mino core protein VP4	-
bluetongue virus serotype 10	-	from infected Spodoptera frugiperda cells via baculovirus	-
bluetongue virus serotype 10	-	i.e. BTV serotype 10	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Spodoptera frugiperda cells after infection with baculovirus, to homogeneity	bluetongue virus serotype 10

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + pp(5')GpXp	-	bluetongue virus serotype 10	G(5')ppp(5')GpXp + diphosphate	guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue	r
GTP + pp(5')RNA	reversible formation of a covalent intermediate enzyme-GMP	bluetongue virus serotype 10	G(5')ppp(5')RNA + diphosphate	guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue	r
GTP + pp(5')RNA	GMP is linked to the enzyme via a phosphoamide bond	bluetongue virus serotype 10	G(5')ppp(5')RNA + diphosphate	guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue	r
additional information	enzyme also catalyzes GTP-diphosphate exchange	bluetongue virus serotype 10	?	-	?

Synonyms

Synonyms	Comment	Organism
More	the capping enzyme complex contains RNA 5'-triphosphatase, RNA guanylyltransferase and pyrophosphatase activities	bluetongue virus serotype 10

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	bluetongue virus serotype 10

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	bluetongue virus serotype 10

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
additional information	-	enzyme-GMP complex is resistant to alkali, but is cleaved at acidic conditions with HCl or NH2OH	bluetongue virus serotype 10

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Release 2023.1 (January 2023)