Activating Compound | Comment | Organism | Structure |
---|---|---|---|
RNA 5'-triphosphatase | binding in the capping enzyme complex stimulates the mRNA guanylyltransferase by 10fold, icreases affinity for GTP | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of wild-type from plasmid in deficient mutant ceg1-25 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | RNA 5'-triphosphatase, CET1 or CES5, expressed from high copy number plasmid in Saccharomyces cerevisiae, can compensate the growth defect caused by mutation ceg1-25 of the RNA guanylyltransferase | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')RNA | Saccharomyces cerevisiae | interaction of RNA 5'-triphosphatase and mRNA guanylyltransferase of the capping enzyme complex is essential for function and viability in vivo | G(5')ppp(5')RNA + diphosphate | - |
? | |
GTP + pp(5')RNA | Saccharomyces cerevisiae | mouse guanylyltransferase domain can substitute the deficient enzyme in Saccharomyces cerevisiae mutant in vivo | G(5')ppp(5')RNA + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
temperature-sensitive growth defect mutant ceg1-25 | - |
Saccharomyces cerevisiae | - |
gene CEG1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')RNA | - |
Saccharomyces cerevisiae | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? | |
GTP + pp(5')RNA | interaction of RNA 5'-triphosphatase and mRNA guanylyltransferase of the capping enzyme complex is essential for function and viability in vivo | Saccharomyces cerevisiae | G(5')ppp(5')RNA + diphosphate | - |
? | |
GTP + pp(5')RNA | mouse guanylyltransferase domain can substitute the deficient enzyme in Saccharomyces cerevisiae mutant in vivo | Saccharomyces cerevisiae | G(5')ppp(5')RNA + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | RNA 5'-triphosphatase and a RNA guanylyltransferase form a heterodimeric complex | Saccharomyces cerevisiae |
More | enzyme is part of the bifunctional capping enzyme, which consists of autonomous and non-overlapping RNA 5'-triphosphatase and a RNA guanylyltransferase domain and activity | Saccharomyces cerevisiae |