Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 50% at 0.005 mM, complete at 0.05 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000019 | - |
diphosphate-ended poly(A) | pH 7.5, 37°C | Homo sapiens | |
0.00014 | - |
lambdac17RNA | pH 7.5, 37°C | Homo sapiens | |
0.000285 | - |
pp(5')ApGp | pH 7.5, 37°C | Homo sapiens | |
0.0011 | - |
GTP | pH 7.5, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
HeLa cells | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | mechanism of capping | Homo sapiens | |
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | mRNA containing a guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dGTP + pp(5')RNA | no activity | Homo sapiens | dG(5')ppp(5')RNA + diphosphate | - |
? | |
GTP + diphosphate-ended poly(A) | - |
Homo sapiens | ? | - |
? | |
GTP + lambdac17RNA | - |
Homo sapiens | ? | - |
? | |
GTP + pp(5')ApGp | - |
Homo sapiens | G(5')ppp(5')ApGp + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ir | |
GTP + pp(5')RNA | the acceptor is diphosphate terminated poly(A) | Homo sapiens | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? | |
GTP + ppp(5')ApGp | - |
Homo sapiens | G(5')pppp(5')ApGp + diphosphate | guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue | ? | |
ITP + pp(5')RNA | - |
Homo sapiens | I(5')ppp(5')RNA + diphosphate | inosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ir | |
additional information | specificity overview | Homo sapiens | ? | - |
? | |
additional information | no activity with GDP | Homo sapiens | ? | - |
? | |
additional information | no activity with m7GTP | Homo sapiens | ? | - |
? | |
additional information | no activity with ATP, CTP, UTP | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |