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Literature summary for 2.7.7.50 extracted from

  • Nishikawa, Y.; Chambon, P.
    Purification of mRNA guanylyltransferase from calf thymus (1982), EMBO J., 1, 485-492.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.50

General Stability

General Stability Organism
can be frozen and thawed several times without apparent loss of activity Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information requirement for divalent cations Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00003
-
 purified enzyme Bos taurus

Storage Stability

Storage Stability Organism
4°C, stable for more than 6 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA the acceptor is diphosphate terminated poly(A) Bos taurus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Bos taurus