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Literature summary for 2.7.7.50 extracted from

  • Shuman, S.; Morham, S.G.
    Domain structure of vaccinia virus mRNA capping enzyme. Activity of the Mr 95,000 subunit expressed in Escherichia coli (1990), J. Biol. Chem., 265, 11967-11972.
    View publication on PubMed
Search for more literature for 2.7.7.50

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vaccinia virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Vaccinia virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 the capping enzyme has 2 subunits: MW 95000 and 31000, the 95000 MW subunit of the vaccinia virus capping enzyme has guanylyltransferase activity, glycerol gradient centrifugation, the isolated 95000 MW guanylyltransferase can be converted to an active 60000 MW form in vitro by limited proteolysis with trypsin, the guanylyltransferase domain is localized to the amino two-thirds of the 95000 MW polypeptide Vaccinia virus

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Vaccinia virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00027
-
-
 Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA
-
 Vaccinia virus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?

Subunits

Subunits Comment Organism
More 95 kDa subunit can be transformed into an active 60 kDa form by trypsin proteolysis Vaccinia virus