Cloned (Comment) | Organism |
---|---|
expression of His-tagged protease domain in Escherichia coli strain BL21 (DE3) pREP4:GroESL | Simian foamy virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azidothymidine | inhibits foamy virus replication | prototype foamy virus | |
azidothymidine | inhibits FV replication | Simian foamy virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0401 | - |
dTTP | determined for TTP on the homopolymeric substrate poly(rA)/oligo(dT), pH and temperature not specified in the publication, recombinant His-tagged protease domain | prototype foamy virus | |
0.045 | - |
deoxynucleoside triphosphate | determined for dNTPs on a heteropolymeric single stranded M13 substrate, pH and temperature not specified in the publication, recombinant His-tagged protease domain | prototype foamy virus | |
0.045 | - |
dTTP | determined for TTP on the homopolymeric substrate poly(rA)/oligo(dT), pH and temperature not specified in the publication, recombinant His-tagged protease domain | Simian foamy virus | |
0.046 | - |
deoxynucleoside triphosphate | determined for dNTPs on a heteropolymeric single stranded M13 substrate, pH and temperature not specified in the publication, recombinant His-tagged protease domain | Simian foamy virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Simian foamy virus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | prototype foamy virus | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
prototype foamy virus | - |
gene pol | - |
Simian foamy virus | - |
gene pol | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protease domain from Escherichia coli strain BL21 (DE3) pREP4:GroESL by nickel affinity and hydrophobic interaction chromatography | Simian foamy virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Simian foamy virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
prototype foamy virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | heteropolymeric single stranded M13 substrate | Simian foamy virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | heteropolymeric single stranded M13 substrate | prototype foamy virus | diphosphate + DNAn+1 | - |
? | |
dTTP + DNAn | homopolymeric substrate poly(rA)/oligo(dT) | Simian foamy virus | diphosphate + DNAn+1 | - |
? | |
dTTP + DNAn | homopolymeric substrate poly(rA)/oligo(dT) | prototype foamy virus | diphosphate + DNAn+1 | - |
? | |
additional information | the N-terminal protease domain of the reverse transcriptase also shows polymerase activity | Simian foamy virus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | hydrophobic interactions might promote dimerization | Simian foamy virus |
dimer | hydrophobic interactions might promote dimerization | prototype foamy virus |
More | the FV reverse transcriptase harbors a protease, polymerase and RNase H domain | Simian foamy virus |
Synonyms | Comment | Organism |
---|---|---|
FV Pol | - |
Simian foamy virus |
FV Pol | - |
prototype foamy virus |
FV reverse transcriptase | - |
Simian foamy virus |
PFV RT | - |
prototype foamy virus |
prototype foamy virus reverse transcriptase | - |
prototype foamy virus |
SFV RT | - |
Simian foamy virus |
simian foamy virus reverse transcriptase | - |
Simian foamy virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
deoxynucleoside triphosphate | determined for dNTPs on a heteropolymeric single stranded M13 substrate, pH and temperature not specified in the publication, recombinant His-tagged protease domain | Simian foamy virus | |
4 | - |
deoxynucleoside triphosphate | determined for dNTPs on a heteropolymeric single stranded M13 substrate, pH and temperature not specified in the publication, recombinant His-tagged protease domain | prototype foamy virus | |
5.5 | - |
dTTP | determined for TTP on the homopolymeric substrate poly(rA)/oligo(dT), pH and temperature not specified in the publication, recombinant His-tagged protease domain | prototype foamy virus | |
7.1 | - |
dTTP | determined for TTP on the homopolymeric substrate poly(rA)/oligo(dT), pH and temperature not specified in the publication, recombinant His-tagged protease domain | Simian foamy virus |