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Literature summary for 2.7.7.49 extracted from
- A modified family-B archaeal DNA polymerase with reverse transcriptase activity (2011), ChemBioChem, 12, 35-37.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D215A | enzyme mutant devoid of 3'-5' exonuclease activity | Thermococcus gorgonarius |
| additional information | Z derivatives have the fingers domain of Tgo-Pol replaced with that from Pol zeta, insertion of the Pol zeta finger domains into the Thermococcus gorgonarius Tgo-Pol resulting in the Z derivatives increase the enzyme activity. Z1 shows increased activity, which is further increased with Z2, created by fusion of Z1 with the processivity-increasing DNA binding protein Sso7d, overview | Thermococcus gorgonarius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Thermococcus gorgonarius | - |
diphosphate + DNAn+1 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus gorgonarius | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Thermococcus gorgonarius | diphosphate + DNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| reverse transcriptase | - |
Thermococcus gorgonarius |
| Tgo-Pol | - |
Thermococcus gorgonarius |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the family B polymerases | Thermococcus gorgonarius |
| malfunction | mutations in a loop in the fingers domain leads to a decrease accuracy of the arhcaeal enzyme | Thermococcus gorgonarius |
| additional information | in family B polymerases, the fingers domain interacts with dNTPs and undergoes a catalytically essential conformational change that depends on correct pairing of the dNTP with its templating base | Thermococcus gorgonarius |
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Release 2023.1 (January 2023)
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