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Literature summary for 2.7.7.49 extracted from

  • Ng, B.; Nayak, S.; Gibbs, M.D.; Lee, J.; Bergquist, P.L.
    Reverse transcriptases: intron-encoded proteins found in thermophilic bacteria (2007), Gene, 393, 137-144.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The recombinant protein proves to be insoluble and is unable to be recovered from the insoluble fraction of lysates of Escherichia coli. The reverse transcriptase is successfully expressed in a baculovirus vector although yields remained low [Bacillus] caldolyticus

Organism

Organism UniProt Comment Textmining
[Bacillus] caldolyticus A2TD24
-
-
[Bacillus] caldolyticus EA1 A2TD24
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant [Bacillus] caldolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn poly(rC)*p(dG)(12-18) [Bacillus] caldolyticus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn poly(rC)*p(dG)(12-18) [Bacillus] caldolyticus EA1 diphosphate + DNAn+1
-
?