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Literature summary for 2.7.7.48 extracted from

  • Dias, A.; Bouvier, D.; Crepin, T.; McCarthy, A.A.; Hart, D.J.; Baudin, F.; Cusack, S.; Ruigrok, R.W.
    The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit (2009), Nature, 458, 914-918.
    View publication on PubMed
Search for more literature for 2.7.7.48

Cloned(Commentary)

Cloned (Comment) Organism
expression of the recombinant enzyme in Escherichia coli influenza A virus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, at 20°C by the hanging drop method using a protein solution of 5-10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl and 2.5 mM MnCl2 and a reservoir composition of 1.2 M Li2SO4, 100 mM MES, pH 6.0, 10 mM Mg acetate and 3% ethylene glycol influenza A virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside triphosphate + RNAn influenza A virus
-
 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn influenza A virus Victoria/3/1975 H3N2
-
 diphosphate + RNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
influenza A virus P31343 subunit P2; Victoria/3/1975, H3N2
-
influenza A virus Victoria/3/1975 H3N2 P31343 subunit P2; Victoria/3/1975, H3N2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by affinity chromatography and gel filtration influenza A virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the polymerase protein also harbors an intrinsic RNA and DNA endonuclease activity that cleaves host mRNAs during cap-snatching, inhibited by 2,4-dioxo-4-phenylbutanoic and activated by Mn2+, with the amino-terminal 209 residues of the PA subunit containing the endonuclease active site, structure-function analysis, overview influenza A virus ?
-
 ?
additional information the polymerase protein also harbors an intrinsic RNA and DNA endonuclease activity that cleaves host mRNAs during cap-snatching, inhibited by 2,4-dioxo-4-phenylbutanoic and activated by Mn2+, with the amino-terminal 209 residues of the PA subunit containing the endonuclease active site, structure-function analysis, overview influenza A virus Victoria/3/1975 H3N2 ?
-
 ?
nucleoside triphosphate + RNAn
-
 influenza A virus diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn various RNA substrates: Alu RNA, 110 nucleotides of the Alu domain of Pyrococcus horikoshii SRP RNA, Candida albicans tRNAAsn, U-rich RNA (59-GGCCAUCCUGU7 CCCU11CU19-39)29, ph-RNA of 81 nucleotides30, and short ph-RNA of 36 nucleotides comprising just the conserved 3' and 5' ends with a short linker and circular single stranded DNA influenza A virus diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn
-
 influenza A virus Victoria/3/1975 H3N2 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn various RNA substrates: Alu RNA, 110 nucleotides of the Alu domain of Pyrococcus horikoshii SRP RNA, Candida albicans tRNAAsn, U-rich RNA (59-GGCCAUCCUGU7 CCCU11CU19-39)29, ph-RNA of 81 nucleotides30, and short ph-RNA of 36 nucleotides comprising just the conserved 3' and 5' ends with a short linker and circular single stranded DNA influenza A virus Victoria/3/1975 H3N2 diphosphate + RNAn+1
-
 ?

Subunits

Subunits Comment Organism
heterotrimer composed of three subunits, PA, PB1 and PB2 influenza A virus

Synonyms

Synonyms Comment Organism
influenza polymerase PA
-
 influenza A virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at influenza A virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at influenza A virus

General Information

General Information Comment Organism
physiological function the enzyme is responsible for replication and transcription of the eight separate segments of the viral RNA genome in the nuclei of infected cells influenza A virus