Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | RdRp phosphorylation by PRK2 is required for activation and RNA replication | Hepacivirus C |
Cloned (Comment) | Organism |
---|---|
expression of NS5B in Huh7 cells | Hepacivirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HA1077 | i.e. fasudil, reduces the levels of intracellular viral RNA by 70% at 0.02 mM, the RdRp activity of HCV NS5B is not inhibited by the PRK2 inhibitor, but its activation by phosphorylation through PRK2 | Hepacivirus C | |
additional information | PRK2 inhibitors can be antiviral drugs that act by suppressing HCV replication via inhibition of viral RNA polymerase phosphorylation | Hepacivirus C | |
Y27632 | blocks HCV RdRp phosphorylation by suppressing PRK2 activation, reduces the levels of intracellular viral RNA by 92% at 0.02 mM, the RdRp activity of HCV NS5B is not inhibited by the PRK2 inhibitor, but its activation by phosphorylation through PRK2 | Hepacivirus C |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
i.e. HCV | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | RdRp phosphorylation by PRK2 is required for activation and RNA replication | Hepacivirus C |
Synonyms | Comment | Organism |
---|---|---|
nonstructural protein 5B | - |
Hepacivirus C |
NS5B | - |
Hepacivirus C |
RDRP | - |
Hepacivirus C |
RNA-dependent RNA polymerase | - |
Hepacivirus C |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Hepacivirus C |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Hepacivirus C |