Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)/pCG1 cells | Enterovirus C |
Crystallization (Comment) | Organism |
---|---|
crystal structure of poliovirus 3CD protein, a virally encoded protease and precursor to the RNA-dependent RNA polymerase | Enterovirus C |
hanging drop vapour diffusion method, with 2 M ammonium sulfate, 0.1 M HEPES (pH 7.0), and 0.3% Jeffamine M600 (pH 7.0) | Enterovirus C |
Protein Variants | Comment | Organism |
---|---|---|
G1A | mutant is only partially active and the N-terminal hydrogen-bonding network is partly disrupted | Enterovirus C |
G64S | inactive | Enterovirus C |
L446D | mutant with disrupt interface I of 3Dpol | Enterovirus C |
R455D | mutant with disrupt interface I of 3Dpol | Enterovirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Superdex 200 gel filtration | Enterovirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Enterovirus C | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3CD protein | precursor of the RNA-dependent RNA polymerase, although 3CD is a fully functional protease, it lacks polymerase activity | Enterovirus C |
3Dpol | - |
Enterovirus C |
RNA-dependent RNA polymerase | - |
Enterovirus C |