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Literature summary for 2.7.7.48 extracted from

  • Cai, Z.; Yi, M.; Zhang, C.; Luo, G.
    Mutagenesis analysis of the rGTP-specific binding site of hepatitis C virus RNA-dependent RNA polymerase (2005), J. Virol., 79, 11607-11617.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hepacivirus C

Protein Variants

Protein Variants Comment Organism
P495A mutation in the rGTP-specific binding site does not cause a decrease in RNA-dependent RNA polymerase in vitro. Mutation decreases RNA replication in the cell Hepacivirus C
P496A mutation in the rGTP-specific binding site does not cause a decrease in RNA-dependent RNA polymerase in vitro. Mutation decreases RNA replication in the cell Hepacivirus C
R32A mutation in the rGTP-specific binding site does not cause a decrease in RNA-dependent RNA polymerase in vitro. Mutation decreases RNA replication in the cell Hepacivirus C
R32K mutation in the rGTP-specific binding site does not cause a decrease in RNA-dependent RNA polymerase in vitro. Mutation decreases RNA replication in the cell Hepacivirus C
R32S mutation in the rGTP-specific binding site does not cause a decrease in RNA-dependent RNA polymerase in vitro. Mutation decreases RNA replication in the cell Hepacivirus C
R503A mutation in the rGTP-specific binding site does not cause a decrease in RNA-dependent RNA polymerase in vitro. Mutation completely ablates RNA replication in the cell Hepacivirus C
S29A mutation in the rGTP-specific binding site does not cause a decrease in RNA-dependent RNA polymerase in vitro. Mutation decreases RNA replication in the cell Hepacivirus C
V499A mutation in the rGTP-specific binding site does not cause a decrease in RNA-dependent RNA polymerase in vitro. Mutation increases RNA replication in cells Hepacivirus C
V499G mutation in the rGTP-specific binding site does not cause a decrease in RNA-dependent RNA polymerase in vitro. Mutation completely ablates RNA replication in the cell Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hepacivirus C

Synonyms

Synonyms Comment Organism
HCV NS5B
-
Hepacivirus C
RDRP
-
Hepacivirus C
RNA-dependent RNA polymerase
-
Hepacivirus C