Cloned (Comment) | Organism |
---|---|
soluble Hepatitis C virus NS5B in the glutathione S-transferase-fused form NS5Bt which lacks the C-terminal 21 amino acid residues, expressed in Escherichia coli | Hepacivirus C |
Protein Variants | Comment | Organism |
---|---|---|
additional information | soluble Hepatitis C virus NS5B in the glutathione S-transferase-fused form NS5Bt which lacks the C-terminal 21 amino acid residues, expressed in Escherichia coli. The recombinant soluble enzyme exhibits RNA-dependent RNA polymerase activity in vitro which is dependent on template and primer, but it does not exhibit the terminal transferase activity | Hepacivirus C |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the C-termimnal region of NS5B, which is dispensable for the RNA-dependent RNA polymerase activity, dramatically affects the subcellular localization of NS5B retaining it in perinuclear sites in transiently overexpressed mammalian cells. The C-terminal region of NS5B, especially the putative anchoring domain has a role in retaining the protein in the cytoplasm | Hepacivirus C | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
soluble Hepatitis C virus NS5B in the glutathione S-transferase-fused form NS5Bt, expressed in Escherichia coli | Hepacivirus C |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00035 | - |
- |
Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? |