Cloned (Comment) | Organism |
---|---|
full-length enzyme and truncated enzyme forms resulting from deletions of 24, 36, 65 or 82 amino acid residues at the C terminal, with C-terminal hexahistidine tag, expression in Escherichia coli | Classical swine fever virus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | truncated enzyme forms resulting from deletions of 24, 36, or amino acid residues at the C terminal have RNA-dependent RNA polymerase activity. Truncated enzyme with a deletion of 82 amino acid residues at the C terminal shows no RNA-dependent RNA polymerase activity. Template specificity of the enzyme is not strict wirth NS5B proteins truncated, suggesting that the C terminal of CSFV NS5B protein is involved in the template specificity of the enzyme | Classical swine fever virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Classical swine fever virus | - |
expression in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
full-length enzyme and truncated enzyme forms resulting from deletions of 24, 36, 65 or 82 amino acid residues at the C terminal, with C-terminal hexahistidine tag | Classical swine fever virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Classical swine fever virus | diphosphate + RNAn+1 | - |
? |