Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heparin | 0.0005 mM, 50% inhibition | Solanum lycopersicum | |
KCl | 100 mM, slight decrease in activity | Solanum lycopersicum | |
NaCl | slight decrease in activity | Solanum lycopersicum | |
NEM | 0.1 mM, reduces the transcription activity down to 10% | Solanum lycopersicum | |
p-Hydroxymercuriphenylsulfonate | 0.1 mM, reduces the transcription activity down to 10% | Solanum lycopersicum | |
Sodium diphosphate | 10 mM, decreases incorporation down to 5% | Solanum lycopersicum | |
spermine | 1 mM, 50% inhibition | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km-value for the equimolar mixture of ATP, CTP and GTP in presence of 0.02 mM UTP is about 0.035 mM | Solanum lycopersicum | |
0.004 | - |
UTP | in presence of 0.3 mM each of ATP, CTP and GTP | Solanum lycopersicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | KCl, activity is increased by a factor of about 1.2-1.5 when the final salt concentration is raised to 60 mM by the addition of NaCl, KCl or NH4Cl | Solanum lycopersicum | |
Mg2+ | maximal activity at 10 mM magnesium acetate | Solanum lycopersicum | |
Mn2+ | optimal activity at 1 mM, 20% of the activity obtained with Mg2+ | Solanum lycopersicum | |
NaCl | activity is increased by a factor of about 1.2-1.5 when the final salt concentration is raised to 60 mM by the addition of NaCl, KCl or NH4Cl | Solanum lycopersicum | |
NH4Cl | activity is increased by a factor of about 1.2-1.5 when the final salt concentration is raised to 60 mM by the addition of NaCl, KCl or NH4Cl | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
119000 | - |
sucrose density gradient centrifugation | Solanum lycopersicum |
128000 | - |
1 * 128000, SDS-PAGE | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
inoculated with potato spindle tuber viroid. In viroid-infected tomato leaf the activity of the host-encoded RdRP is significantly increased. Viroids are not translated into proteins so that they cannot code for a viroid-specific RNA replicase | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | leaf inoculated with potato spindle tuber viroid. In viroid-infected tomato leaf the activity of the host-encoded RdRP is significantly increased. Viroids are not translated into proteins so that they cannot code for a viroid-specific RNA replicase | Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0166 | - |
- |
Solanum lycopersicum |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol-containing buffer, activity decreases by about 15% per month | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Solanum lycopersicum | diphosphate + RNAn+1 | - |
? | |
UTP + RNAn | - |
Solanum lycopersicum | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 128000, SDS-PAGE | Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Solanum lycopersicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | 37 | 27°C: about 50% of maximal activity, 37°C: optimum | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7.0: about 70% of maximal activity, pH 9.0: about 40% of maximal activity | Solanum lycopersicum |