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Literature summary for 2.7.7.48 extracted from

  • Schiebel, W.; Haas, B.; Marinkovic, S.; Klanner, A.; Sänger, H.L.
    RNA-directed RNA polymerase from tomato leaves. I. Purification and physical properties (1993), J. Biol. Chem., 268, 11851-11857.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
heparin 0.0005 mM, 50% inhibition Solanum lycopersicum
KCl 100 mM, slight decrease in activity Solanum lycopersicum
NaCl slight decrease in activity Solanum lycopersicum
NEM 0.1 mM, reduces the transcription activity down to 10% Solanum lycopersicum
p-Hydroxymercuriphenylsulfonate 0.1 mM, reduces the transcription activity down to 10% Solanum lycopersicum
Sodium diphosphate 10 mM, decreases incorporation down to 5% Solanum lycopersicum
spermine 1 mM, 50% inhibition Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the Km-value for the equimolar mixture of ATP, CTP and GTP in presence of 0.02 mM UTP is about 0.035 mM Solanum lycopersicum
0.004
-
UTP in presence of 0.3 mM each of ATP, CTP and GTP Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ KCl, activity is increased by a factor of about 1.2-1.5 when the final salt concentration is raised to 60 mM by the addition of NaCl, KCl or NH4Cl Solanum lycopersicum
Mg2+ maximal activity at 10 mM magnesium acetate Solanum lycopersicum
Mn2+ optimal activity at 1 mM, 20% of the activity obtained with Mg2+ Solanum lycopersicum
NaCl activity is increased by a factor of about 1.2-1.5 when the final salt concentration is raised to 60 mM by the addition of NaCl, KCl or NH4Cl Solanum lycopersicum
NH4Cl activity is increased by a factor of about 1.2-1.5 when the final salt concentration is raised to 60 mM by the addition of NaCl, KCl or NH4Cl Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
119000
-
sucrose density gradient centrifugation Solanum lycopersicum
128000
-
1 * 128000, SDS-PAGE Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
inoculated with potato spindle tuber viroid. In viroid-infected tomato leaf the activity of the host-encoded RdRP is significantly increased. Viroids are not translated into proteins so that they cannot code for a viroid-specific RNA replicase
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf leaf inoculated with potato spindle tuber viroid. In viroid-infected tomato leaf the activity of the host-encoded RdRP is significantly increased. Viroids are not translated into proteins so that they cannot code for a viroid-specific RNA replicase Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0166
-
-
Solanum lycopersicum

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol-containing buffer, activity decreases by about 15% per month Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn
-
Solanum lycopersicum diphosphate + RNAn+1
-
?
UTP + RNAn
-
Solanum lycopersicum diphosphate + RNAn+1
-
?

Subunits

Subunits Comment Organism
monomer 1 * 128000, SDS-PAGE Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Solanum lycopersicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
27 37 27°C: about 50% of maximal activity, 37°C: optimum Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 70% of maximal activity, pH 9.0: about 40% of maximal activity Solanum lycopersicum