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Literature summary for 2.7.7.48 extracted from

  • Clark, G.L.; Peden, K.W.C.; Symons, R.H.
    Cucumber mosaic virus-induced RNA polymerase: partial purification and properties of the template-free enzyme (1974), Virology, 62, 434-444.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
pancreatic RNase 0.006 mg/ml, marked inhibition Cucumber mosaic virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration is 13 mM Cucumber mosaic virus

Organism

Organism UniProt Comment Textmining
Cucumber mosaic virus
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cucumber cotyledons infected with
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Purification (Commentary)

Purification (Comment) Organism
partial Cucumber mosaic virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00088
-
reaction with CMV RNA Cucumber mosaic virus
0.0015
-
reaction with poly(C) Cucumber mosaic virus

Storage Stability

Storage Stability Organism
-15°C, buffer containing 30% glycerol, complete loss of activity after 2-3 days Cucumber mosaic virus
0°C, buffer containing 30% glycerol, complete loss of activity after 1-2 days Cucumber mosaic virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn GTP + CMV RNA, yeast RNA or poly(C) Cucumber mosaic virus diphosphate + RNAn+1
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