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Literature summary for 2.7.7.48 extracted from

  • Blumenthal, T.
    Qbeta replicase (1982), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 15, 267-279.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
host factor hexameric host-coded protein of 12500 Da subunits is required for in vitro Qbeta RNA replication Qubevirus durum

Inhibitors

Inhibitors Comment Organism Structure
aurintricarboxylic acid inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains Qubevirus durum
GTP competitive inhibitor of initiation but not of elongation Qubevirus durum
Poly(U) inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains Qubevirus durum
Polyethylene sulfonate inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains Qubevirus durum
ppGpp competitive inhibitor of initiation but not of elongation Qubevirus durum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template Qubevirus durum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts Qubevirus durum
130000
-
the low value compared to the sum of molecular weights of the four subunits is probably a result of an oblate shape conferred by ribosomal protein S1, glycerol gradient sedimentation Qubevirus durum
215000
-
SDS-PAGE of four polypeptides covalently cross-linked Qubevirus durum
450000
-
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts Qubevirus durum
650000
-
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts Qubevirus durum
700000
-
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts Qubevirus durum

Organism

Organism UniProt Comment Textmining
Qubevirus durum
-
Escherichia coli infected with
-
Qubevirus durum
-
QbetaamB86
-
Qubevirus durum
-
Qbetaam12
-
Qubevirus durum Qbetaam12
-
Qbetaam12
-
Qubevirus durum QbetaamB86
-
QbetaamB86
-

Purification (Commentary)

Purification (Comment) Organism
-
Qubevirus durum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template Qubevirus durum diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn GTP and polyC Qubevirus durum diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template Qubevirus durum Qbetaam12 diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn GTP and polyC Qubevirus durum Qbetaam12 diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template Qubevirus durum QbetaamB86 diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn GTP and polyC Qubevirus durum QbetaamB86 diphosphate + RNAn+1
-
?

Subunits

Subunits Comment Organism
tetramer x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts Qubevirus durum