Activating Compound | Comment | Organism | Structure |
---|---|---|---|
host factor | hexameric host-coded protein of 12500 Da subunits is required for in vitro Qbeta RNA replication | Qubevirus durum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aurintricarboxylic acid | inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains | Qubevirus durum | |
GTP | competitive inhibitor of initiation but not of elongation | Qubevirus durum | |
Poly(U) | inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains | Qubevirus durum | |
Polyethylene sulfonate | inhibits by competing with the template for binding to the enzyme, does not inhibit the elongation of preinitiated RNA chains | Qubevirus durum | |
ppGpp | competitive inhibitor of initiation but not of elongation | Qubevirus durum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template | Qubevirus durum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts | Qubevirus durum |
130000 | - |
the low value compared to the sum of molecular weights of the four subunits is probably a result of an oblate shape conferred by ribosomal protein S1, glycerol gradient sedimentation | Qubevirus durum |
215000 | - |
SDS-PAGE of four polypeptides covalently cross-linked | Qubevirus durum |
450000 | - |
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts | Qubevirus durum |
650000 | - |
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts | Qubevirus durum |
700000 | - |
x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts | Qubevirus durum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Qubevirus durum | - |
Escherichia coli infected with | - |
Qubevirus durum | - |
QbetaamB86 | - |
Qubevirus durum | - |
Qbetaam12 | - |
Qubevirus durum Qbetaam12 | - |
Qbetaam12 | - |
Qubevirus durum QbetaamB86 | - |
QbetaamB86 | - |
Purification (Comment) | Organism |
---|---|
- |
Qubevirus durum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template | Qubevirus durum | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | GTP and polyC | Qubevirus durum | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template | Qubevirus durum Qbetaam12 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | GTP and polyC | Qubevirus durum Qbetaam12 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | synthesis of RNA in response to RNA template. An RNA primer can substitute for GTP to allow initiation. Mn2+ might reduce the template specificity by forming a complex with GTP that is more efficiently incorporated than is Mg*GTP with unfavored template | Qubevirus durum QbetaamB86 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | GTP and polyC | Qubevirus durum QbetaamB86 | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | x * 35000 + x * 450000 + x * 650000 + x * 700000, the enzyme is composed of 4 nonidentical subunits, only the 65000 Da subunit is phage-coded. The 70000 Da protein is 30S ribosomal protein S1 and the other two are the protein synthesis elongation factors Tu and Ts | Qubevirus durum |