BRENDA - Enzyme Database
show all sequences of 2.7.7.46

Determination of the rate-limiting segment of aminoglycoside nucleotidyltransferase 2''-I by pH- and viscosity-dependent kinetics

Gates, C.A.; Northrop, D.B.; Biochemistry 27, 3834-3842 (1988)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
gentamycin C1
-
Escherichia coli
netilmicin
substrate inhibition
Escherichia coli
sisomicin
uncompetitive substrate inhibition
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + netilmicin C
-
645290
Escherichia coli
diphosphate + 2''-adenylylnetilmicin C
-
-
-
r
ATP + sisomicin
-
645290
Escherichia coli
diphosphate + 2''-adenylylsisomicin
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
aminoglycoside nucleotidyltransferase 2''-I
-
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9.5
-
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.154
-
sisomicin
-
Escherichia coli
1.84
-
gentamycin C1
-
Escherichia coli
5.12
-
netilmicin
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
gentamycin C1
-
Escherichia coli
netilmicin
substrate inhibition
Escherichia coli
sisomicin
uncompetitive substrate inhibition
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.154
-
sisomicin
-
Escherichia coli
1.84
-
gentamycin C1
-
Escherichia coli
5.12
-
netilmicin
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + netilmicin C
-
645290
Escherichia coli
diphosphate + 2''-adenylylnetilmicin C
-
-
-
r
ATP + sisomicin
-
645290
Escherichia coli
diphosphate + 2''-adenylylsisomicin
-
-
-
r
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9.5
-
Escherichia coli
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
645292
Ekman
Cloning, overexpression, and p ...
Pseudomonas aeruginosa
Biochemistry
40
7017-7024
2001
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645291
Van Pelt
1H, 13C, and 31P nuclear magne ...
Escherichia coli
Arch. Biochem. Biophys.
280
284-291
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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4
-
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-
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4
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
645289
Gates
Alternative substrate and inhi ...
Escherichia coli, Escherichia coli W677/pMY10
Biochemistry
27
3826-3833
1988
-
1
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
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11
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
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3
-
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-
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-
-
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11
-
-
-
-
-
-
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-
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-
-
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-
645290
Gates
Determination of the rate-limi ...
Escherichia coli
Biochemistry
27
3834-3842
1988
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
645286
Van Pelt
Purification and properties of ...
Escherichia coli
Arch. Biochem. Biophys.
230
250-263
1984
-
-
-
-
-
-
7
7
-
1
2
-
-
2
-
-
1
-
-
-
2
-
7
1
2
-
-
-
-
1
1
-
-
7
2
-
-
-
-
-
-
-
-
-
7
7
7
-
1
2
-
-
-
-
1
-
-
2
-
7
1
-
-
-
-
1
1
-
2
-
-
-
-
-
-
637300
Angelatou
Purification and properties of ...
Pseudomonas aeruginosa
J. Antibiot.
35
235-244
1982
-
1
-
-
-
1
1
-
-
3
-
1
-
3
-
-
1
-
-
-
-
1
9
-
-
-
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-
-
1
-
-
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-
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1
-
-
-
-
1
-
1
-
-
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3
-
1
-
-
-
1
-
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1
9
-
-
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-
1
-
-
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-
-
-
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645287
Yagisawa
-
Adenylyldideoxykanamycin B, a ...
Escherichia coli, Escherichia coli JR66/W677
J. Antibiot.
26
911-912
1971
-
-
-
-
-
-
-
-
1
1
-
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-
3
-
-
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-
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-
2
-
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-
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1
1
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-
2
-
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-
-
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645288
Naganawa
The structure determination of ...
Escherichia coli, Escherichia coli JR66/W677
J. Antibiot.
26
913-914
1971
-
-
-
-
-
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-
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3
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2
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2
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